| Relationship between antibacterial activity of chitosan and surface characteristics of cell wall. | |
| | |
MedLine Citation:
|
PMID: 15210068 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
AIM: Five representative waterborne pathogens were used to illustrate the relationship between chitosan's antibacterial activity and the surface characteristics of the bacterial cell wall. METHODS: Chitosan was prepared with averaged 75% or 95% deacetylated degree to examine its antibacterial activity against waterborne pathogens. Fresh microbial inoculants for the antibacterial assessment were prepared on nutrient agar at 37 degrees for 24 h. The evaluation items of antibacterial mechanism included hydrophilicity and negative charge analysis of cell surface, and adsorptive characteristics of chitosan to bacterial cell. All the experiments were applied in triplicate tests at least. RESULTS: Although cell wall hydrophilicity was similar among Gram-negative bacteria, the distribution of negative charge on their cell surfaces was quite different. More negatively charged cell surfaces had a greater interaction with chitosan, a phenomenon further confirmed by transmission electron micrography (TEM). CONCLUSION: Results showed the hydrophilicity in Gram-negative bacteria was much higher than in Gram-positive ones. The correlation coefficient 0.988 between the amount of absorbed chitosan and its inhibition efficiency indicated a close relationship. |
| | |
Authors:
|
Ying-chien Chung; Ya-ping Su; Chiing-chang Chen; Guang Jia; Huey-lan Wang; J C Gaston Wu; Jaung-geng Lin |
Related Documents
:
|
8553018 - Comparison of cell sediment and surface grown "test plaque" using scanning electron mic... 20442418 - In vivo identification of regulators of cell invasion across basement membranes. 17345138 - Pseudomonas fluorescens sbw25 biofilm and planktonic cells have differentiable raman sp... 11207558 - Enteropathogenic escherichia coli (epec) attachment to epithelial cells: exploiting the... 20962878 - Phage-induced lysis enhances biofilm formation in shewanella oneidensis mr-1. 22511878 - Cell contact-dependent outer membrane exchange in myxobacteria: genetic determinants an... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Acta pharmacologica Sinica Volume: 25 ISSN: 1671-4083 ISO Abbreviation: Acta Pharmacol. Sin. Publication Date: 2004 Jul |
Date Detail:
|
Created Date: 2004-06-22 Completed Date: 2004-08-31 Revised Date: 2004-11-17 |
Medline Journal Info:
|
Nlm Unique ID: 100956087 Medline TA: Acta Pharmacol Sin Country: China |
Other Details:
|
Languages: eng Pagination: 932-6 Citation Subset: IM |
Affiliation:
|
Department of Industrial Engineering and Management, China Institute of Technology, Taipei 245, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adsorption Anti-Bacterial Agents / metabolism, pharmacology* Cell Wall / chemistry, physiology Chitin / analogs & derivatives*, metabolism, pharmacology* Chitosan Electrophysiology Enterococcus faecalis / physiology Escherichia coli / physiology Gram-Negative Bacteria / physiology* Gram-Positive Bacteria / physiology* Pseudomonas aeruginosa / physiology Salmonella typhimurium / physiology Staphylococcus aureus / physiology Surface Properties / drug effects* |
| Chemical | |
Reg. No./Substance:
|
0/Anti-Bacterial Agents; 1398-61-4/Chitin; 9012-76-4/Chitosan |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Imrecoxib: a novel and selective cyclooxygenase 2 inhibitor with anti-inflammatory effect.
Next Document: Anticancer effect of two diterpenoid compounds isolated from Annona glabra Linn.