Document Detail

Relationship between adipose stromal-vascular cells and adipocytes in human adipose tissue.
MedLine Citation:
PMID:  8744503     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To determine the relative amounts of RNA and DNA from adipocytes and adipose stromal-vascular cells to better assess differential gene expression for estrogen receptor and cytochrome P450 aromatase in the two cell fractions of adipose tissue.
STUDY DESIGN: Colorimetric and fluorometric assays were used to measure total RNA and DNA, and the relative cell numbers as well as the relative contribution of RNA from adipocytes and stromal-vascular cells were assessed.
RESULTS: Adipocytes and stromal-vascular cells exist in a ratio of 2/1. RNA/DNA ratios are the same in the two cell fractions.
CONCLUSION: In human subcutaneous adipose tissue, adipocytes are twice as numerous as stromal-vascular cells. The overall transcriptional activity of the cell types appears similar.
S N O'Brien; K A Mantzke; M W Kilgore; T M Price
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Analytical and quantitative cytology and histology / the International Academy of Cytology [and] American Society of Cytology     Volume:  18     ISSN:  0884-6812     ISO Abbreviation:  Anal. Quant. Cytol. Histol.     Publication Date:  1996 Apr 
Date Detail:
Created Date:  1996-09-27     Completed Date:  1996-09-27     Revised Date:  2014-11-05    
Medline Journal Info:
Nlm Unique ID:  8506819     Medline TA:  Anal Quant Cytol Histol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  137-43     Citation Subset:  IM    
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MeSH Terms
Adipocytes / metabolism*
Adipose Tissue / metabolism*,  pathology
Aromatase / genetics*
Cell Count
Cytochrome P-450 Enzyme System
DNA / metabolism
Gene Expression
Middle Aged
RNA / metabolism
Receptors, Estrogen / genetics*
Stromal Cells / metabolism
Reg. No./Substance:
0/Receptors, Estrogen; 63231-63-0/RNA; 9007-49-2/DNA; 9035-51-2/Cytochrome P-450 Enzyme System; EC

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