| Relationship between adipose stromal-vascular cells and adipocytes in human adipose tissue. | |
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MedLine Citation:
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PMID: 8744503 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To determine the relative amounts of RNA and DNA from adipocytes and adipose stromal-vascular cells to better assess differential gene expression for estrogen receptor and cytochrome P450 aromatase in the two cell fractions of adipose tissue. STUDY DESIGN: Colorimetric and fluorometric assays were used to measure total RNA and DNA, and the relative cell numbers as well as the relative contribution of RNA from adipocytes and stromal-vascular cells were assessed. RESULTS: Adipocytes and stromal-vascular cells exist in a ratio of 2/1. RNA/DNA ratios are the same in the two cell fractions. CONCLUSION: In human subcutaneous adipose tissue, adipocytes are twice as numerous as stromal-vascular cells. The overall transcriptional activity of the cell types appears similar. |
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Authors:
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S N O'Brien; K A Mantzke; M W Kilgore; T M Price |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Analytical and quantitative cytology and histology / the International Academy of Cytology [and] American Society of Cytology Volume: 18 ISSN: 0884-6812 ISO Abbreviation: Anal. Quant. Cytol. Histol. Publication Date: 1996 Apr |
Date Detail:
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Created Date: 1996-09-27 Completed Date: 1996-09-27 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 8506819 Medline TA: Anal Quant Cytol Histol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 137-43 Citation Subset: IM |
Affiliation:
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Greenville Hospital System/Clemson University Biomedical Cooperative Program, Clemson University, South Carolina 29634-1912, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adipocytes
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metabolism* Adipose Tissue / metabolism*, pathology Adult Aromatase / genetics* Calorimetry Cell Count Cytochrome P-450 Enzyme System DNA / metabolism Female Fluorometry Gene Expression Humans Middle Aged RNA / metabolism Receptors, Estrogen / genetics* Stromal Cells / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Receptors, Estrogen; 63231-63-0/RNA; 9007-49-2/DNA; 9035-51-2/Cytochrome P-450 Enzyme System; EC 1.14.14.1/Aromatase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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