Document Detail


Relationship between abnormal sperm morphology induced by dietary zinc deficiency and lipid composition in testes of growing rats.
MedLine Citation:
PMID:  19222874     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The present study investigated the effect of dietary Zn deficiency during sexual maturation on sperm integrity and testis phospholipid fatty acid composition. Male weanling Sprague-Dawley rats were randomised into four dietary groups for 3 weeks: Zn control (ZC; 30 mg Zn/kg); Zn marginally deficient (ZMD; 9 mg Zn/kg); Zn deficient (ZD; < 1 mg Zn/kg); pair fed (PF; 30 mg Zn/kg) to the ZD group. Morphology of cauda epididymal sperm and lipid profiles of testis phospholipids were analysed. The rats fed the ZD diet had a lower testis weight (P < 0.02). Seminal vesicles and prostate weight were also lower in the ZD and PF groups. Rats fed the ZD diet, but not the ZMD diet, had 34-35 % more abnormal spermatozoa and 24 % shorter sperm tail length than the ZC and PF rats (P < 0.001). Testis cholesterol concentration was higher in the ZD rats compared with the ZC and PF rats (P < 0.04). Testes were highly enriched with n-6 fatty acids by showing n-6 : n-3 fatty acid ratios of 27:1 in phosphatidylcholine (PC) and 23:1 in phosphatidylethanolamine (PE). The dominant fatty acid in testes was docosapentaenoic acid (22 : 5n-6), comprising 15 and 24 % of PC and PE, respectively. This fatty acid was significantly lower in the ZD rats, whereas 18 : 2n-6 was higher compared with the rats in the other diet groups. These results demonstrate that severe Zn deficiency adversely affects sperm integrity and modulates testis fatty acid composition by interrupting essential fatty acid metabolism. This suggests that Zn deficiency-associated abnormal testicular function is perhaps preceded by altered membrane fatty acid composition, especially of a major fatty acid, 22 : 5n-6.
Authors:
Krystal J Merrells; Heather Blewett; Jennifer A Jamieson; Carla G Taylor; Miyoung Suh
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-02-18
Journal Detail:
Title:  The British journal of nutrition     Volume:  102     ISSN:  1475-2662     ISO Abbreviation:  Br. J. Nutr.     Publication Date:  2009 Jul 
Date Detail:
Created Date:  2009-07-09     Completed Date:  2009-09-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372547     Medline TA:  Br J Nutr     Country:  England    
Other Details:
Languages:  eng     Pagination:  226-32     Citation Subset:  IM    
Affiliation:
Department of Human Nutritional Sciences, University of Manitoba, Winnipeg, Manitoba R3T2N2, Canada.
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MeSH Terms
Descriptor/Qualifier:
Animal Feed
Animals
Biological Markers / blood
Body Weight
Cholesterol / blood
Diet*
Fatty Acids, Unsaturated / analysis*
Male
Nutritional Status
Organ Size
Phosphatidylcholines / chemistry
Phosphatidylethanolamines / chemistry
Rats
Rats, Sprague-Dawley
Sexual Maturation
Spermatozoa / pathology*
Testis / chemistry*,  pathology
Triglycerides / analysis
Weaning
Zinc / deficiency*,  metabolism
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Fatty Acids, Unsaturated; 0/Phosphatidylcholines; 0/Phosphatidylethanolamines; 0/Triglycerides; 39382-08-6/phosphatidylethanolamine; 57-88-5/Cholesterol; 7440-66-6/Zinc

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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