| Relationship between abnormal sperm morphology induced by dietary zinc deficiency and lipid composition in testes of growing rats. | |
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MedLine Citation:
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PMID: 19222874 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The present study investigated the effect of dietary Zn deficiency during sexual maturation on sperm integrity and testis phospholipid fatty acid composition. Male weanling Sprague-Dawley rats were randomised into four dietary groups for 3 weeks: Zn control (ZC; 30 mg Zn/kg); Zn marginally deficient (ZMD; 9 mg Zn/kg); Zn deficient (ZD; < 1 mg Zn/kg); pair fed (PF; 30 mg Zn/kg) to the ZD group. Morphology of cauda epididymal sperm and lipid profiles of testis phospholipids were analysed. The rats fed the ZD diet had a lower testis weight (P < 0.02). Seminal vesicles and prostate weight were also lower in the ZD and PF groups. Rats fed the ZD diet, but not the ZMD diet, had 34-35 % more abnormal spermatozoa and 24 % shorter sperm tail length than the ZC and PF rats (P < 0.001). Testis cholesterol concentration was higher in the ZD rats compared with the ZC and PF rats (P < 0.04). Testes were highly enriched with n-6 fatty acids by showing n-6 : n-3 fatty acid ratios of 27:1 in phosphatidylcholine (PC) and 23:1 in phosphatidylethanolamine (PE). The dominant fatty acid in testes was docosapentaenoic acid (22 : 5n-6), comprising 15 and 24 % of PC and PE, respectively. This fatty acid was significantly lower in the ZD rats, whereas 18 : 2n-6 was higher compared with the rats in the other diet groups. These results demonstrate that severe Zn deficiency adversely affects sperm integrity and modulates testis fatty acid composition by interrupting essential fatty acid metabolism. This suggests that Zn deficiency-associated abnormal testicular function is perhaps preceded by altered membrane fatty acid composition, especially of a major fatty acid, 22 : 5n-6. |
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Authors:
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Krystal J Merrells; Heather Blewett; Jennifer A Jamieson; Carla G Taylor; Miyoung Suh |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2009-02-18 |
Journal Detail:
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Title: The British journal of nutrition Volume: 102 ISSN: 1475-2662 ISO Abbreviation: Br. J. Nutr. Publication Date: 2009 Jul |
Date Detail:
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Created Date: 2009-07-09 Completed Date: 2009-09-03 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372547 Medline TA: Br J Nutr Country: England |
Other Details:
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Languages: eng Pagination: 226-32 Citation Subset: IM |
Affiliation:
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Department of Human Nutritional Sciences, University of Manitoba, Winnipeg, Manitoba R3T2N2, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animal Feed Animals Biological Markers / blood Body Weight Cholesterol / blood Diet* Fatty Acids, Unsaturated / analysis* Male Nutritional Status Organ Size Phosphatidylcholines / chemistry Phosphatidylethanolamines / chemistry Rats Rats, Sprague-Dawley Sexual Maturation Spermatozoa / pathology* Testis / chemistry*, pathology Triglycerides / analysis Weaning Zinc / deficiency*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/Fatty Acids, Unsaturated; 0/Phosphatidylcholines; 0/Phosphatidylethanolamines; 0/Triglycerides; 39382-08-6/phosphatidylethanolamine; 57-88-5/Cholesterol; 7440-66-6/Zinc |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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