| The relationship between retinal ganglion cell axon constituents and retinal nerve fiber layer birefringence in the primate. | |
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MedLine Citation:
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PMID: 19494208 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: To determine the degree of correlation between spatial characteristics of the retinal nerve fiber layer (RNFL) birefringence (Delta n(RNFL)) surrounding the optic nerve head (ONH) with the corresponding anatomy of retinal ganglion cell (RGC) axons and their respective organelles. METHODS: RNFL phase retardation per unit depth (PR/UD, proportional to Delta n(RNFL)) was measured in two cynomolgus monkeys by enhanced polarization-sensitive optical coherence tomography (EPS-OCT). The monkeys were perfused with glutaraldehyde and the eyes were enucleated and prepared for transmission electron microscopy (TEM) histologic analysis. Morphologic measurements from TEM images were used to estimate neurotubule density (rho(RNFL)), axoplasmic area (A(x)) mode, axon area (A(a)) mode, slope (u) of the number of neurotubules versus axoplasmic area (neurotubule packing density), fractional area of axoplasm in the nerve fiber bundle (f), mitochondrial fractional area in the nerve fiber bundle (x(m)), mitochondria-containing axon profile fraction (m(p)), and length of axonal membrane profiles per unit of nerve fiber bundle area (L(am)/A(b)). Registered PR/UD and morphologic parameters from corresponding angular sections were then correlated by using Pearson's correlation and multilevel models. RESULTS: In one eye there was a statistically significant correlation between PR/UD and rho(RNFL) (r = 0.67, P = 0.005) and between PR/UD and neurotubule packing density (r = 0.70, P = 0.002). Correlation coefficients of r = 0.81 (P = 0.01) and r = 0.50 (P = 0.05) were observed between the PR/UD and A(x) modes for each respective subject. CONCLUSIONS: Neurotubules are the primary source of birefringence in the RNFL of the primate retina. |
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Authors:
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Ginger M Pocock; Roberto G Aranibar; Nate J Kemp; Charles S Specht; Mia K Markey; H Grady Rylander |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2009-06-03 |
Journal Detail:
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Title: Investigative ophthalmology & visual science Volume: 50 ISSN: 1552-5783 ISO Abbreviation: Invest. Ophthalmol. Vis. Sci. Publication Date: 2009 Nov |
Date Detail:
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Created Date: 2009-11-03 Completed Date: 2009-12-04 Revised Date: 2011-09-26 |
Medline Journal Info:
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Nlm Unique ID: 7703701 Medline TA: Invest Ophthalmol Vis Sci Country: United States |
Other Details:
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Languages: eng Pagination: 5238-46 Citation Subset: IM |
Affiliation:
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Biomedical Engineering Laser Laboratories, Department of Biomedical Engineering, The University of Texas at Austin, Austin, Texas 78712, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Axons / physiology*, ultrastructure Birefringence Female Macaca fascicularis Microtubules / physiology* Nerve Fibers / physiology*, ultrastructure Optic Disk / physiology*, ultrastructure Organelles Retinal Ganglion Cells / physiology*, ultrastructure Tomography, Optical Coherence |
| Grant Support | |
ID/Acronym/Agency:
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R01 EY016462-04/EY/NEI NIH HHS; R01EY016462-01A1/EY/NEI NIH HHS |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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