| Relationship between MALDI IMS intensity and measured quantity of selected phospholipids in rat brain sections. | |
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MedLine Citation:
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PMID: 20853893 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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MALDI IMS positive ion images of rat brain show a regional distribution of phosphocholine species that is striking in the apparent distinctiveness and reproducibility of such depictions. The interpretation of these images, specifically the relationship between MALDI IMS ion intensity and the amount of the phosphocholine (PC) species in the tissue is complicated by numerous factors, such as ion suppression, ion molecule chemistry, and effects of tissue structure. This study was designed to test the hypothesis that the intensity of PC molecular species does relate to the quantity of molecules in a tissue sample. A set of comparison studies for a limited but representative selection of cell-derived PC molecular species was carried out using LC/MS/MS to measure the amounts of these species in brain tissue extracts. There was good correlation between the MALDI IMS ion abundance of PC molecular species and the relative abundance of corresponding PC molecular species in microdissected regions analyzed by LC/MS/MS. |
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Authors:
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Joseph A Hankin; Robert C Murphy |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Analytical chemistry Volume: 82 ISSN: 1520-6882 ISO Abbreviation: Anal. Chem. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2011-04-15 Completed Date: 2011-04-29 Revised Date: 2012-10-09 |
Medline Journal Info:
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Nlm Unique ID: 0370536 Medline TA: Anal Chem Country: United States |
Other Details:
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Languages: eng Pagination: 8476-84 Citation Subset: IM |
Affiliation:
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Department of Pharmacology, University of Colorado Denver, Mail Stop 8303, Aurora, Colorado 80045, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Brain Chemistry* Female Phospholipids / analysis* Rats Rats, Sprague-Dawley Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods* |
| Grant Support | |
ID/Acronym/Agency:
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GM069338/GM/NIGMS NIH HHS; R24 AA013162/AA/NIAAA NIH HHS; U01 AA016663/AA/NIAAA NIH HHS; U54 GM069338/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Phospholipids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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