Document Detail


Relationship between Egr-1 gene expression and apoptosis in esophageal carcinoma and precancerous lesions.
MedLine Citation:
PMID:  12439908     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: To study the expression of early growth response gene-1 (Egr-1 gene) and Bcl-X/(L) protein and its relationship with the cell apoptosis in human esophageal carcinoma (EC) and precancerous lesions. METHODS: In situ hybridization(ISH), immunohistochemistry (IHC) and TUNEL method were used respectively to detect Egr-1mRNA, Egr-1 protein, apoptosis related-protein Bcl-X/(L) and cell apoptosis in situ from 66 cases of esophageal squamous cell carcinoma and their upper cut edge and paracancerous mucosa. RESULTS: Egr-1 gene in situ hybridization, Bcl-X/(L) immunohistochemistry positive products were located in the cytoplasm, while Egr-1 immunohistochemistry and TUNEL positive signal were located in the nuclei. The apoptosis index(AI) and the frequency of apoptosis occurrence were increased gradually from precancerous lesion to cancer (P<0.01) and the expression of Egr-1mRNA and Egr-1 protein in dysplasia was the highest among all specimens (P<0.01). The AI of Egr-1 positive cancer tissues was much higher than that of Egr-1 negative cancer tissues (P<0.01), while the AI of Bcl-X/(L) positive cancer tissues was much lower than that of Bcl-X/(L) negative cancer tissues (P<0.01). The AI and Egr-1 expression were not correlated with invasiveness and lymphatic metastasis in EC. CONCLUSION: Cell apoptosis was present through esophageal carcinogenesis. The expression of Egr-1 mRNA and Egr-1 protein were high in precancerous lesion of esophagus. The AI was increased significantly in Egr-1 positive squamous cell carcinoma. Egr-1 might promote apoptotic effect. Egr-1 expression and cell apoptosis may have an important biological significance in esophageal carcinogenesis.
Authors:
Ming-Yao Wu; Ying-Rui Liang; Xian-Ying Wu; Chu-Xiang Zhuang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  World journal of gastroenterology : WJG     Volume:  8     ISSN:  1007-9327     ISO Abbreviation:  World J. Gastroenterol.     Publication Date:  2002 Dec 
Date Detail:
Created Date:  2002-11-19     Completed Date:  2003-01-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100883448     Medline TA:  World J Gastroenterol     Country:  China    
Other Details:
Languages:  eng     Pagination:  971-5     Citation Subset:  IM    
Affiliation:
Department of Pathology, Shantou University Medical College, Guangdong Province, China. mywu@stu.edu.cn
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MeSH Terms
Descriptor/Qualifier:
Apoptosis / genetics*
Carcinoma, Squamous Cell / genetics*,  metabolism,  pathology*
DNA-Binding Proteins / genetics*,  metabolism
Early Growth Response Protein 1
Esophageal Neoplasms / genetics*,  metabolism,  pathology*
Gene Expression
Humans
Immediate-Early Proteins*
Immunohistochemistry
In Situ Hybridization
In Situ Nick-End Labeling
Precancerous Conditions / genetics*,  metabolism,  pathology*
Proto-Oncogene Proteins c-bcl-2 / metabolism
RNA, Messenger / genetics,  metabolism
RNA, Neoplasm / genetics,  metabolism
Transcription Factors / genetics*,  metabolism
bcl-X Protein
Chemical
Reg. No./Substance:
0/BCL2L1 protein, human; 0/DNA-Binding Proteins; 0/EGR1 protein, human; 0/Early Growth Response Protein 1; 0/Immediate-Early Proteins; 0/Proto-Oncogene Proteins c-bcl-2; 0/RNA, Messenger; 0/RNA, Neoplasm; 0/Transcription Factors; 0/bcl-X Protein

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