| Relationship between Egr-1 gene expression and apoptosis in esophageal carcinoma and precancerous lesions. | |
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MedLine Citation:
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PMID: 12439908 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIM: To study the expression of early growth response gene-1 (Egr-1 gene) and Bcl-X/(L) protein and its relationship with the cell apoptosis in human esophageal carcinoma (EC) and precancerous lesions. METHODS: In situ hybridization(ISH), immunohistochemistry (IHC) and TUNEL method were used respectively to detect Egr-1mRNA, Egr-1 protein, apoptosis related-protein Bcl-X/(L) and cell apoptosis in situ from 66 cases of esophageal squamous cell carcinoma and their upper cut edge and paracancerous mucosa. RESULTS: Egr-1 gene in situ hybridization, Bcl-X/(L) immunohistochemistry positive products were located in the cytoplasm, while Egr-1 immunohistochemistry and TUNEL positive signal were located in the nuclei. The apoptosis index(AI) and the frequency of apoptosis occurrence were increased gradually from precancerous lesion to cancer (P<0.01) and the expression of Egr-1mRNA and Egr-1 protein in dysplasia was the highest among all specimens (P<0.01). The AI of Egr-1 positive cancer tissues was much higher than that of Egr-1 negative cancer tissues (P<0.01), while the AI of Bcl-X/(L) positive cancer tissues was much lower than that of Bcl-X/(L) negative cancer tissues (P<0.01). The AI and Egr-1 expression were not correlated with invasiveness and lymphatic metastasis in EC. CONCLUSION: Cell apoptosis was present through esophageal carcinogenesis. The expression of Egr-1 mRNA and Egr-1 protein were high in precancerous lesion of esophagus. The AI was increased significantly in Egr-1 positive squamous cell carcinoma. Egr-1 might promote apoptotic effect. Egr-1 expression and cell apoptosis may have an important biological significance in esophageal carcinogenesis. |
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Authors:
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Ming-Yao Wu; Ying-Rui Liang; Xian-Ying Wu; Chu-Xiang Zhuang |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: World journal of gastroenterology : WJG Volume: 8 ISSN: 1007-9327 ISO Abbreviation: World J. Gastroenterol. Publication Date: 2002 Dec |
Date Detail:
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Created Date: 2002-11-19 Completed Date: 2003-01-30 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 100883448 Medline TA: World J Gastroenterol Country: China |
Other Details:
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Languages: eng Pagination: 971-5 Citation Subset: IM |
Affiliation:
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Department of Pathology, Shantou University Medical College, Guangdong Province, China. mywu@stu.edu.cn |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis
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genetics* Carcinoma, Squamous Cell / genetics*, metabolism, pathology* DNA-Binding Proteins / genetics*, metabolism Early Growth Response Protein 1 Esophageal Neoplasms / genetics*, metabolism, pathology* Gene Expression Humans Immediate-Early Proteins* Immunohistochemistry In Situ Hybridization In Situ Nick-End Labeling Precancerous Conditions / genetics*, metabolism, pathology* Proto-Oncogene Proteins c-bcl-2 / metabolism RNA, Messenger / genetics, metabolism RNA, Neoplasm / genetics, metabolism Transcription Factors / genetics*, metabolism bcl-X Protein |
| Chemical | |
Reg. No./Substance:
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0/BCL2L1 protein, human; 0/DNA-Binding Proteins; 0/EGR1 protein, human; 0/Early Growth Response Protein 1; 0/Immediate-Early Proteins; 0/Proto-Oncogene Proteins c-bcl-2; 0/RNA, Messenger; 0/RNA, Neoplasm; 0/Transcription Factors; 0/bcl-X Protein |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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