| Relationship between Ca2+ sparklets and sarcoplasmic reticulum Ca2+ load and release in rat cerebral arterial smooth muscle. | |
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MedLine Citation:
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PMID: 21984539 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Ca(+) sparklets are subcellular Ca(2+) signals produced by the opening of sarcolemmal L-type Ca(2+) channels. Ca(2+) sparklet activity varies within the sarcolemma of arterial myocytes. In this study, we examined the relationship between Ca(2+) sparklet activity and sarcoplasmic reticulum (SR) Ca(2+) accumulation and release in cerebral arterial myocytes. Our data indicate that the SR is a vast organelle with multiple regions near the sarcolemma of these cells. Ca(2+) sparklet sites were located at or <0.2 μm from SR-sarcolemmal junctions. We found that while Ca(2+) sparklets increase the rate of SR Ca(2+) refilling in arterial myocytes, their activity did not induce regional variations in SR Ca(2+) content or Ca(2+) spark activity. In arterial myocytes, L-type Ca(2+) channel activity was independent of SR Ca(2+) load. This ruled out a potential feedback mechanism whereby SR Ca(2+) load regulates the activity of these channels. Together, our data suggest a model in which Ca(2+) sparklets contribute Ca(2+) influx into a cytosolic Ca(2+) pool from which sarco(endo)plasmic reticulum Ca(2+)-ATPase pumps Ca(2+) into the SR, indirectly regulating SR function. |
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Authors:
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Yukari Takeda; Matthew A Nystoriak; Madeline Nieves-Cintrón; Luis F Santana; Manuel F Navedo |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2011-10-07 |
Journal Detail:
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Title: American journal of physiology. Heart and circulatory physiology Volume: 301 ISSN: 1522-1539 ISO Abbreviation: Am. J. Physiol. Heart Circ. Physiol. Publication Date: 2011 Dec |
Date Detail:
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Created Date: 2011-12-06 Completed Date: 2012-01-23 Revised Date: 2013-02-19 |
Medline Journal Info:
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Nlm Unique ID: 100901228 Medline TA: Am J Physiol Heart Circ Physiol Country: United States |
Other Details:
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Languages: eng Pagination: H2285-94 Citation Subset: IM |
Affiliation:
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Department of Physiology and Biophysics, University of Washington, Seattle, 98195, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Calcium / metabolism* Calcium Channels, L-Type / metabolism Calcium Signaling* Cerebral Arteries / metabolism Luminescent Proteins / biosynthesis, genetics Microscopy, Confocal Microscopy, Fluorescence Muscle, Smooth, Vascular / cytology, metabolism* Myocytes, Smooth Muscle / metabolism* Protein Sorting Signals Rats Rats, Sprague-Dawley Recombinant Fusion Proteins / biosynthesis Ryanodine Receptor Calcium Release Channel / metabolism Sarcolemma / metabolism* Sarcoplasmic Reticulum / metabolism* Sarcoplasmic Reticulum Calcium-Transporting ATPases / metabolism Time Factors Transfection |
| Grant Support | |
ID/Acronym/Agency:
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HL-085686/HL/NHLBI NIH HHS; HL-085870/HL/NHLBI NIH HHS; HL-098200/HL/NHLBI NIH HHS; R01 HL098200/HL/NHLBI NIH HHS; T32-HL-07828/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Calcium Channels, L-Type; 0/Luminescent Proteins; 0/Protein Sorting Signals; 0/Recombinant Fusion Proteins; 0/Ryanodine Receptor Calcium Release Channel; 0/red fluorescent protein; 7440-70-2/Calcium; EC 3.6.3.8/Sarcoplasmic Reticulum Calcium-Transporting ATPases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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