Document Detail


Relationship between Ca2+ sparklets and sarcoplasmic reticulum Ca2+ load and release in rat cerebral arterial smooth muscle.
MedLine Citation:
PMID:  21984539     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ca(+) sparklets are subcellular Ca(2+) signals produced by the opening of sarcolemmal L-type Ca(2+) channels. Ca(2+) sparklet activity varies within the sarcolemma of arterial myocytes. In this study, we examined the relationship between Ca(2+) sparklet activity and sarcoplasmic reticulum (SR) Ca(2+) accumulation and release in cerebral arterial myocytes. Our data indicate that the SR is a vast organelle with multiple regions near the sarcolemma of these cells. Ca(2+) sparklet sites were located at or <0.2 μm from SR-sarcolemmal junctions. We found that while Ca(2+) sparklets increase the rate of SR Ca(2+) refilling in arterial myocytes, their activity did not induce regional variations in SR Ca(2+) content or Ca(2+) spark activity. In arterial myocytes, L-type Ca(2+) channel activity was independent of SR Ca(2+) load. This ruled out a potential feedback mechanism whereby SR Ca(2+) load regulates the activity of these channels. Together, our data suggest a model in which Ca(2+) sparklets contribute Ca(2+) influx into a cytosolic Ca(2+) pool from which sarco(endo)plasmic reticulum Ca(2+)-ATPase pumps Ca(2+) into the SR, indirectly regulating SR function.
Authors:
Yukari Takeda; Matthew A Nystoriak; Madeline Nieves-Cintrón; Luis F Santana; Manuel F Navedo
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-10-07
Journal Detail:
Title:  American journal of physiology. Heart and circulatory physiology     Volume:  301     ISSN:  1522-1539     ISO Abbreviation:  Am. J. Physiol. Heart Circ. Physiol.     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-12-06     Completed Date:  2012-01-23     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  100901228     Medline TA:  Am J Physiol Heart Circ Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  H2285-94     Citation Subset:  IM    
Affiliation:
Department of Physiology and Biophysics, University of Washington, Seattle, 98195, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Calcium / metabolism*
Calcium Channels, L-Type / metabolism
Calcium Signaling*
Cerebral Arteries / metabolism
Luminescent Proteins / biosynthesis,  genetics
Microscopy, Confocal
Microscopy, Fluorescence
Muscle, Smooth, Vascular / cytology,  metabolism*
Myocytes, Smooth Muscle / metabolism*
Protein Sorting Signals
Rats
Rats, Sprague-Dawley
Recombinant Fusion Proteins / biosynthesis
Ryanodine Receptor Calcium Release Channel / metabolism
Sarcolemma / metabolism*
Sarcoplasmic Reticulum / metabolism*
Sarcoplasmic Reticulum Calcium-Transporting ATPases / metabolism
Time Factors
Transfection
Grant Support
ID/Acronym/Agency:
HL-085686/HL/NHLBI NIH HHS; HL-085870/HL/NHLBI NIH HHS; HL-098200/HL/NHLBI NIH HHS; R01 HL098200/HL/NHLBI NIH HHS; T32-HL-07828/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Calcium Channels, L-Type; 0/Luminescent Proteins; 0/Protein Sorting Signals; 0/Recombinant Fusion Proteins; 0/Ryanodine Receptor Calcium Release Channel; 0/red fluorescent protein; 7440-70-2/Calcium; EC 3.6.3.8/Sarcoplasmic Reticulum Calcium-Transporting ATPases
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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