| Relationship between 201Tl uptake and oxidative metabolism in cultured myocardial cells. | |
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MedLine Citation:
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PMID: 3714145 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The uptake of thallium-201 (201Tl) by myocardial cells in cultures was assessed in the presence of 10(-3) M potassium cyanide (KCN), an inhibitor of mitochondrial respiration, and 10(-4) M dinitrophenol (DNP), an uncoupler of oxidative phosphorylation. The cultures were incubated with 14C-glucose or 14C-octanoate, allowing the measurement of the oxidative metabolism and beta-oxidation from the production of 14CO2. The results demonstrated a moderate decrease in the ratio between the intra/extracellular concentration of 201Tl (Tl i/e) in the presence of KCN (28.9 +/- 8.1 versus 35.6 +/- 9.7 in the controls, n.s.) and no change with DNP (37.6 +/- 9.7). Glycolysis and fatty acid oxidation were lowered with KCN (-28 +/- 15 and -45 +/- 22% respectively, p less than 0.05 in both cases) and were non significantly increased with DNP (+37 +/- 23 and +10 +/- 52% respectively). These results show that 201Tl intracellular uptake is not related directly, but is not totally independent of glycolysis and fatty acid oxidation. |
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Authors:
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P Gachon; N Moins; J C Maublant; M R Ross; W D Davidson; I Mena |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Nuclear medicine communications Volume: 7 ISSN: 0143-3636 ISO Abbreviation: Nucl Med Commun Publication Date: 1986 Jan |
Date Detail:
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Created Date: 1986-07-09 Completed Date: 1986-07-09 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8201017 Medline TA: Nucl Med Commun Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 59-64 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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2,4-Dinitrophenol Cells, Cultured Culture Media Dinitrophenols / pharmacology Heart / radionuclide imaging Humans Myocardium / cytology, metabolism* Oxidation-Reduction Oxidative Phosphorylation / drug effects Oxygen Consumption / drug effects Potassium Cyanide / pharmacology Radioisotopes / diagnostic use* Thallium / diagnostic use* Uncoupling Agents / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/Dinitrophenols; 0/Radioisotopes; 0/Uncoupling Agents; 151-50-8/Potassium Cyanide; 51-28-5/2,4-Dinitrophenol; 7440-28-0/Thallium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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