Document Detail


Relationship between 201Tl uptake and oxidative metabolism in cultured myocardial cells.
MedLine Citation:
PMID:  3714145     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The uptake of thallium-201 (201Tl) by myocardial cells in cultures was assessed in the presence of 10(-3) M potassium cyanide (KCN), an inhibitor of mitochondrial respiration, and 10(-4) M dinitrophenol (DNP), an uncoupler of oxidative phosphorylation. The cultures were incubated with 14C-glucose or 14C-octanoate, allowing the measurement of the oxidative metabolism and beta-oxidation from the production of 14CO2. The results demonstrated a moderate decrease in the ratio between the intra/extracellular concentration of 201Tl (Tl i/e) in the presence of KCN (28.9 +/- 8.1 versus 35.6 +/- 9.7 in the controls, n.s.) and no change with DNP (37.6 +/- 9.7). Glycolysis and fatty acid oxidation were lowered with KCN (-28 +/- 15 and -45 +/- 22% respectively, p less than 0.05 in both cases) and were non significantly increased with DNP (+37 +/- 23 and +10 +/- 52% respectively). These results show that 201Tl intracellular uptake is not related directly, but is not totally independent of glycolysis and fatty acid oxidation.
Authors:
P Gachon; N Moins; J C Maublant; M R Ross; W D Davidson; I Mena
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Nuclear medicine communications     Volume:  7     ISSN:  0143-3636     ISO Abbreviation:  Nucl Med Commun     Publication Date:  1986 Jan 
Date Detail:
Created Date:  1986-07-09     Completed Date:  1986-07-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8201017     Medline TA:  Nucl Med Commun     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  59-64     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
2,4-Dinitrophenol
Cells, Cultured
Culture Media
Dinitrophenols / pharmacology
Heart / radionuclide imaging
Humans
Myocardium / cytology,  metabolism*
Oxidation-Reduction
Oxidative Phosphorylation / drug effects
Oxygen Consumption / drug effects
Potassium Cyanide / pharmacology
Radioisotopes / diagnostic use*
Thallium / diagnostic use*
Uncoupling Agents / pharmacology
Chemical
Reg. No./Substance:
0/Culture Media; 0/Dinitrophenols; 0/Radioisotopes; 0/Uncoupling Agents; 151-50-8/Potassium Cyanide; 51-28-5/2,4-Dinitrophenol; 7440-28-0/Thallium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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