Document Detail


Relationship of actin, microtubules, and crosswall synthesis during septation in Aspergillus nidulans.
MedLine Citation:
PMID:  9415379     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Studies of cytokinesis in animal cells demonstrate that microtubules play an important role in signaling the position of the actin-containing contractile ring and subsequent formation of the cleavage furrow. Septation in several fungi closely resembles animal cell cytokinesis in that a circumferential ring of actin is visible at the incipient division site. However, this does not necessarily mean that division is contractile since actin may also serve to localize septal wall synthesis. In addition, several studies in fission yeast have suggested that microtubules are dispensable for actin ring formation. We have used synchronized cells and fluorescence microscopy to follow actin structures, nuclear division and septal wall synthesis during septation in Aspergillus nidulans. Our data suggest that actin first appears at the septum site as a circumferential ring and that it later broadens and invaginates, forming an hourglass-shaped structure coincident with septal cell wall synthesis. Depolymerization of microtubules early in septation prevents circumferential actin ring formation. Depolymerization of microtubules after circumferential actin ring formation blocks both the progression to invaginating bands and septal wall synthesis. In contrast to studies in yeast cells, our data suggest that microtubules are required for both the initiation and progression of septation in A. nidulans.
Authors:
M Momany; J E Hamer
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cell motility and the cytoskeleton     Volume:  38     ISSN:  0886-1544     ISO Abbreviation:  Cell Motil. Cytoskeleton     Publication Date:  1997  
Date Detail:
Created Date:  1998-02-12     Completed Date:  1998-02-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8605339     Medline TA:  Cell Motil Cytoskeleton     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  373-84     Citation Subset:  IM    
Affiliation:
Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907-1392, USA. momany@dogwood.botany.uga.edu
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MeSH Terms
Descriptor/Qualifier:
Actins / physiology*
Aspergillus nidulans / metabolism,  physiology*,  ultrastructure
Cell Division / physiology
Cell Wall / physiology
Chitin / metabolism
Microtubules / physiology*
Chemical
Reg. No./Substance:
0/Actins; 1398-61-4/Chitin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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