Document Detail


Relations between cyclin D1 and chromosome 11 in thyroid carcinoma: analysis by dual stain using FISH and immunostaining method.
MedLine Citation:
PMID:  19443989     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cyclin D1 is an important cell-cycle regulator that drives the cell cycle from the G1 phase to the S phase. Elevated nuclear cyclin D1 expression has been found in human tumors, including thyroid carcinoma. Protein production is known to require DNA amplification in each cell, but reports of such amplification have not been published. This study aimed to analyze the relationship between cyclin D1 protein production and chromosome 11 in cultured cells by means of dual staining with fluorescence in situ hybridaization (FISH) and immunostaining. In addition, we immunostained anaplastic thyroid carcinoma tissue. The results indicate that cyclin D1 is not related to chromosome 11 in cultured cells. Furthermore, tissue study showed that cyclin D1 is produced in the cytoplasm and in nuclei in various ratios.
Authors:
Tomoo Jikuzono; Toshihiro Tenjin; Kazuo Shimizu
Related Documents :
340939 - Localization of gamma-rays induced chromatid breaks using a three consecutive staining ...
669699 - Are nors easily translocated to deleted chromosomes?
10997779 - Combined pi-dapi staining (cpd) reveals nor asymmetry and facilitates karyotyping of pl...
14667039 - Argyrophilic nucleolar organizer regions (agnors) in interphases and metaphases of norm...
2416669 - Simultaneous production of r-bands and either replication patterns or sister chromatid ...
2431619 - Detecting abnormal human chromosome constitutions by dual laser flow cytogenetics.
11908659 - A microsatellite sequence from the rice blast fungus (magnaporthe grisea) distinguishes...
15124229 - Loss-of-imprinting of peg1 in mouse interspecies hybrids is correlated with altered gro...
8100139 - Dp polymorphism in hla-a1,-b8,-dr3 extended haplotypes associated with membranoprolifer...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of Nippon Medical School = Nippon Ika Daigaku zasshi     Volume:  76     ISSN:  1345-4676     ISO Abbreviation:  J Nippon Med Sch     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2009-05-15     Completed Date:  2009-06-30     Revised Date:  2012-09-25    
Medline Journal Info:
Nlm Unique ID:  100935589     Medline TA:  J Nippon Med Sch     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  54-5     Citation Subset:  IM    
Affiliation:
Department of Biological Regulation, Graduate School of Medicine, Nippon Medical School, Tokyo, Japan. t-jikuzono@nms.ac.jp
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Carcinoma / genetics*,  metabolism,  pathology
Cell Cycle
Chromosomes, Human, Pair 11*
Cyclin D1 / metabolism*
G1 Phase
Gene Expression Regulation, Neoplastic*
Humans
Immunohistochemistry / methods*
In Situ Hybridization, Fluorescence*
S Phase
Staining and Labeling / methods*
Thyroid Neoplasms / genetics*,  metabolism,  pathology
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/CCND1 protein, human; 136601-57-5/Cyclin D1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Epidural Abscess Caused by Haemophilus aphrophilus Misidentified as Pasteurella Species.
Next Document:  Adipose-derived stem cells for tissue repair and regeneration: ten years of research and a literatur...