Document Detail

Relations between cyclin D1 and chromosome 11 in thyroid carcinoma: analysis by dual stain using FISH and immunostaining method.
MedLine Citation:
PMID:  19443989     Owner:  NLM     Status:  MEDLINE    
Cyclin D1 is an important cell-cycle regulator that drives the cell cycle from the G1 phase to the S phase. Elevated nuclear cyclin D1 expression has been found in human tumors, including thyroid carcinoma. Protein production is known to require DNA amplification in each cell, but reports of such amplification have not been published. This study aimed to analyze the relationship between cyclin D1 protein production and chromosome 11 in cultured cells by means of dual staining with fluorescence in situ hybridaization (FISH) and immunostaining. In addition, we immunostained anaplastic thyroid carcinoma tissue. The results indicate that cyclin D1 is not related to chromosome 11 in cultured cells. Furthermore, tissue study showed that cyclin D1 is produced in the cytoplasm and in nuclei in various ratios.
Tomoo Jikuzono; Toshihiro Tenjin; Kazuo Shimizu
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of Nippon Medical School = Nippon Ika Daigaku zasshi     Volume:  76     ISSN:  1345-4676     ISO Abbreviation:  J Nippon Med Sch     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2009-05-15     Completed Date:  2009-06-30     Revised Date:  2012-09-25    
Medline Journal Info:
Nlm Unique ID:  100935589     Medline TA:  J Nippon Med Sch     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  54-5     Citation Subset:  IM    
Department of Biological Regulation, Graduate School of Medicine, Nippon Medical School, Tokyo, Japan.
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MeSH Terms
Carcinoma / genetics*,  metabolism,  pathology
Cell Cycle
Chromosomes, Human, Pair 11*
Cyclin D1 / metabolism*
G1 Phase
Gene Expression Regulation, Neoplastic*
Immunohistochemistry / methods*
In Situ Hybridization, Fluorescence*
S Phase
Staining and Labeling / methods*
Thyroid Neoplasms / genetics*,  metabolism,  pathology
Tumor Cells, Cultured
Reg. No./Substance:
0/CCND1 protein, human; 136601-57-5/Cyclin D1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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