Document Detail


Relation of the segregative origin of chromosome replication to the origin of replication after amino acid starvation.
MedLine Citation:
PMID:  4891256     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cultures of Escherichia coli 15T(-) and K-12 were labeled with (3)H-thymine before, during, and after amino acid starvation. The number of labeled segregating units was measured by autoradiography of microcolonies derived from the labeled cells. In both strains, labels inserted before starvation and during starvation appeared to segregate as if incorporated into the same polynucleotide strands. However, labels inserted during and after starvation segregated as if incorporated into different polynucleotide strands. In view of previous data, it was concluded that replication after amino acid starvation originates from the region of the chromosome which serves as the origin for replication during normal growth and division.
Authors:
H Eberle; K G Lark
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of bacteriology     Volume:  98     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1969 May 
Date Detail:
Created Date:  1969-07-30     Completed Date:  1969-07-30     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  536-42     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Autoradiography
Chromosomes, Bacterial*
DNA Replication
DNA, Bacterial / biosynthesis
Escherichia coli*
Genetics, Microbial*
Thymidine / metabolism
Tritium
Chemical
Reg. No./Substance:
0/DNA, Bacterial; 10028-17-8/Tritium; 50-89-5/Thymidine
Comments/Corrections

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