Document Detail


Relation between in vivo and in vitro measurements of skeletal muscle oxidative metabolism.
MedLine Citation:
PMID:  11745976     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The relationships between in vivo (31)P magnetic resonance spectroscopy (MRS) and in vitro markers of oxidative capacity (mitochondrial function) were determined in 27 women with varying levels of physical fitness. Following 90-s isometric plantar flexion exercises, calf muscle mitochondrial function was determined from the phosphocreatine (PCr) recovery time constant, the adenosine diphosphate (ADP) recovery time constant, the rate of change of PCr during the initial 14 s of recovery, and the apparent maximum rate of oxidative adenosine triphosphate (ATP) synthesis (Q(max)). Muscle fiber type distribution (I, IIa, IIx), citrate synthase (CS) activity, and cytochrome c oxidase (COX) activity were determined from a biopsy sample of lateral gastrocnemius. MRS markers of mitochondrial function correlated moderately (P < 0.05) with the percentage of type IIa oxidative fibers (r = 0.41 to 0.66) and CS activity (r = 0.48 to 0.64), but only weakly with COX activity (r = 0.03 to 0.26, P > 0.05). These results support the use of MRS to determine mitochondrial function in vivo.
Authors:
D E Larson-Meyer; B R Newcomer; G R Hunter; D R Joanisse; R L Weinsier; M M Bamman
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Publication Detail:
Type:  Comparative Study; In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Muscle & nerve     Volume:  24     ISSN:  0148-639X     ISO Abbreviation:  Muscle Nerve     Publication Date:  2001 Dec 
Date Detail:
Created Date:  2001-12-17     Completed Date:  2002-01-09     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7803146     Medline TA:  Muscle Nerve     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1665-76     Citation Subset:  IM    
Copyright Information:
Copyright 2001 John Wiley & Sons, Inc.
Affiliation:
Pennington Biomedical Research Center, Louisiana State University, 6400 Perkins Road, Baton Rouge, Louisiana 70808-4124, USA. larsonde@pbrc.edu
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MeSH Terms
Descriptor/Qualifier:
Adenosine Diphosphate / metabolism
Adenosine Triphosphate / metabolism
Adult
Biopsy
Citrate (si)-Synthase / metabolism
Electron Transport Complex IV / metabolism
Female
Humans
Isometric Contraction / physiology
Magnetic Resonance Imaging
Middle Aged
Mitochondria / metabolism*
Muscle, Skeletal / cytology,  metabolism*
Oxygen Consumption / physiology*
Phosphocreatine / metabolism
Grant Support
ID/Acronym/Agency:
DK-49779/DK/NIDDK NIH HHS; MO1-RR-00032/RR/NCRR NIH HHS; P30-DK56336/DK/NIDDK NIH HHS; R01 DK-51684/DK/NIDDK NIH HHS; RR 11811/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
56-65-5/Adenosine Triphosphate; 58-64-0/Adenosine Diphosphate; 67-07-2/Phosphocreatine; EC 1.9.3.1/Electron Transport Complex IV; EC 2.3.3.1/Citrate (si)-Synthase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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