Document Detail

Relation between house-dust endotoxin exposure, type 1 T-cell development, and allergen sensitisation in infants at high risk of asthma.
MedLine Citation:
PMID:  10905243     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Bacterial endotoxin is known to induce interferon gamma and interleukin 12 production, and therefore has the potential to decrease allergen sensitisation. To find out the role of early chronic endotoxin exposure in the development of allergen sensitisation and asthma, we compared concentrations of endotoxin in house dust with allergen sensitisation in infants at high risk for developing asthma. METHODS: 61 infants 9-24 months old with at least three physician-documented episodes of wheezing were studied. Concentrations of house-dust endotoxin and allergens were measured in the infants' homes. Allergen sensitisation was measured by skin-prick testing with a panel of common inhalant and food allergens. In a subset of these infants, proportions of T lymphocytes producing interferon gamma, and interleukins 4, 5, and 13 were calculated by cell-surface and intracellular cytokine staining, with flow cytometry. FINDINGS: House-dust endotoxin concentrations ranged from 104 to 10,000 endotoxin units (EU) per mL (geometric mean 912 EU/mL). Concentrations did not vary significantly over a 6-month interval. Ten infants (16%) were sensitised to at least one allergen. The homes of allergen-sensitised infants contained significantly lower concentrations of house-dust endotoxin than those of non-sensitised infants (mean 468 vs 1035 EU/mL, respectively; p=0.01). Increased house-dust endotoxin concentrations correlated with increased proportions of interferon-gamma-producing CD4 T cells (p=0.01). Such concentrations did not correlate with proportions of cells that produced interleukins 4, 5, or 13. INTERPRETATION: This study may provide the first direct in-vivo evidence that indoor endotoxin exposure early in life may protect against allergen sensitisation by enhancing type 1 immunity.
J E Gereda; D Y Leung; A Thatayatikom; J E Streib; M R Price; M D Klinnert; A H Liu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Lancet     Volume:  355     ISSN:  0140-6736     ISO Abbreviation:  Lancet     Publication Date:  2000 May 
Date Detail:
Created Date:  2000-08-01     Completed Date:  2000-08-01     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985213R     Medline TA:  Lancet     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1680-3     Citation Subset:  AIM; IM; X    
Division of Pediatric Allergy & Immunology, National Jewish Medical & Research Center, Denver, CO 80206, USA.
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MeSH Terms
Allergens / immunology*
Asthma / immunology*,  prevention & control
CD4-CD8 Ratio
Child, Preschool
Endotoxins / immunology*
Hypersensitivity, Immediate / immunology*,  prevention & control
Intradermal Tests
Respiratory Hypersensitivity / immunology*,  prevention & control
Risk Factors
T-Lymphocytes / immunology*
Grant Support
Reg. No./Substance:
0/Allergens; 0/Endotoxins
Comment In:
Lancet. 2000 Sep 30;356(9236):1191-2   [PMID:  11030320 ]
Lancet. 2000 Aug 5;356(9228):506   [PMID:  10981912 ]

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