| Relation between enzyme release and irreversible cell injury of the heart under the influence of cytoskeleton modulating agents. | |
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MedLine Citation:
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PMID: 9540843 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effects of agents modulating the cytoskeleton, taxol (microtubuli stabilizing), vinblastine (microtubuli destabilizing) and cytochalasin D (actin destabilizing) (10(-6) M each) on enzyme and ATP release as well as on irreversible cell injury were investigated in isolated perfused hypoxic and reoxygenated rat hearts. Enzyme (creatine kinase (CK)) and ATP concentration were assayed in the interstitial transudate and venous effluent. Irreversible cell injury was determined from trypan blue uptake and nuclear staining (NS) of cardiomyocytes in histologic sections. ATP release from nonneuronal cells was only detectable in the interstitial transudate and was not significantly altered by the agents. In controls total CK release (about 4% of total CK) exceeded the percentage of irreversibly injured cells by a factor of 8. Taxol and cytochalasin D abolished the hypoxia/reoxygenation induced interstitial CK release and reduced total CK release to a highly significant extent. The percentage of irreversible injured cells was even more diminished by these agents resulting in a ratio of CK/NS of 40. The effect of cytochalasin D apparently is the consequence of decreased contractile performance as shown by analogous depression by butonedione monoxine (BDM), whereas contractile activity was not altered by taxol. Vinblastine had no influence on CK release but increased the number of irreversibly injured cells significantly. In conclusion, cytoskeletal elements apparently participate in the hypoxia/reoxygenation induced process of release of cytosolic enzymes (CK) and irreversible injury in a different way and extent. Taxol exhibits a cytoprotective effect in isolated perfused rat hearts as evaluated by the extent of enzyme release and irreversible cell injury. |
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Authors:
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E Skobel; H Kammermeier |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 1362 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1997 Dec |
Date Detail:
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Created Date: 1998-04-21 Completed Date: 1998-04-21 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 128-34 Citation Subset: IM |
Affiliation:
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Institut für Physiologie der Medizinischen, Fakultät der RWTH Aachen, Universitätsklinikum, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphate
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metabolism Animals Anoxia / metabolism Creatine Kinase / metabolism Cytochalasin D / pharmacology Cytoskeleton / drug effects* Female Heart Rate Myocardial Contraction / drug effects Myocardial Reperfusion Injury / enzymology*, pathology Myocardium / enzymology* Paclitaxel / pharmacology Rats Rats, Sprague-Dawley Systole Vinblastine / pharmacology |
| Chemical | |
Reg. No./Substance:
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22144-77-0/Cytochalasin D; 33069-62-4/Paclitaxel; 56-65-5/Adenosine Triphosphate; 865-21-4/Vinblastine; EC 2.7.3.2/Creatine Kinase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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