Document Detail


Relation between RLP-triglyceride to RLP-cholesterol ratio and particle size distribution in RLP-cholesterol profiles by HPLC.
MedLine Citation:
PMID:  10807977     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Remnant-Like Particles (RLP) isolated by an immunoseparation method are heterogeneous in their physical and biochemical properties. The objective of this study was to examine the relation between RLP-triglyceride (RLP-TG) to RLP-cholesterol (RLP-C) ratio and particle size distribution in RLP-C profiles from patients with hyperlipoproteinemia by HPLC. RLP were isolated from serum samples from 147 subjects. RLP-C and RLP-TG were quantified by respective enzymatic methods. Particle sizes of the RLP were measured using HPLC with 4 connected TSKgel LipopropakXL columns. Based on HPLC profiles of RLP-C from individual subjects, three different types were classified: predominantly LDL, predominantly VLDL, and mostly VLDL types. All patients with type III hyperlipidemia were mostly VLDL type but with smaller particle size of VLDL (32 nm) than other subjects. Severe hypertriglyceridemic (TG>4.52 mmoll(-1)) subjects were mostly VLDL type with large particle size (41 nm). As for all subjects (n=105) without predominantly LDL type, a significant correlation between RLP particle size and RLP-TG to RLP-C ratio (r=0. 432, P<0.001) was obtained, but not in case of serum TG to RLP-C ratio (r=0.062). It suggests that RLP-TG to RLP-C ratio might be used for discrimination of atherogenic smaller-sized lipoprotein from larger-sized TG-rich lipoprotein remnants.
Authors:
M Okazaki; S Usui; N Tada; T Nakano; K Nakajima
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinica chimica acta; international journal of clinical chemistry     Volume:  296     ISSN:  0009-8981     ISO Abbreviation:  Clin. Chim. Acta     Publication Date:  2000 Jun 
Date Detail:
Created Date:  2000-07-14     Completed Date:  2000-07-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1302422     Medline TA:  Clin Chim Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  135-49     Citation Subset:  IM    
Affiliation:
Laboratory of Chemistry, College of Liberal Arts and Sciences, Tokyo Medical and Dental University, 2-8-30, Kohnodai, Ichikawa-shi, Chiba, Japan. okazaki.cul@cul.tmd.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Apolipoproteins / blood*
Cholesterol*
Chromatography, High Pressure Liquid
Humans
Hyperlipoproteinemia Type II / blood
Hyperlipoproteinemia Type III / blood
Hyperlipoproteinemia Type IV / blood
Hyperlipoproteinemias / blood
Hypertriglyceridemia / blood
Lipoproteins / blood*
Lipoproteins, LDL / blood
Lipoproteins, VLDL / blood
Particle Size
Triglycerides / blood*
Chemical
Reg. No./Substance:
0/Apolipoproteins; 0/Lipoproteins; 0/Lipoproteins, LDL; 0/Lipoproteins, VLDL; 0/Triglycerides; 0/remnant-like particle cholesterol; 57-88-5/Cholesterol

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