Document Detail


[Relation between PMI and FTIR spectral changes in asphyxiated rat's liver and spleen].
MedLine Citation:
PMID:  23213779     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
OBJECTIVE: Fourier transform infrared (FTIR) spectroscopy was applied to observe the postmortem degradation process in mechanical asphyxiated rat's liver and spleen for providing a new method of estimating PMI.
METHODS: Rats were sacrificed by mechanical asphyxia and cadavers were kept at (20 +/- 2) degrees C in a control chamber. The liver and spleen were sub-sampled from the same rat at intervals of 0-15 days postmortem and the data were measured by FTIR spectrometer. The different absorbance (A) ratios of peaks were calculated and the curve estimation analysis between absorbance ratios (x) and PMI (y) were performed to establish mathematical models by the statistical software.
RESULTS: The band absorbance ratios showed increase, decrease and stable with PMI. The cubic model functions showed the strongest correlation coefficient. Compared with the spleen, the liver showed a higher correlation coefficient. The A1541/A1396 of liver showed the highest correlation coefficient (r=0.966). After 6-7 days postmortem, band absorbance ratios showed a steady period.
CONCLUSION: FTIR spectroscopy can be a new and efficient method to estimate PMI within 7 days.
Authors:
Shi-ying Li; Yu Shao; Zheng-dong Li; Dong-hua Zou; Zhi-qiang Qin; Yi-jiu Chen; Ping Huang
Publication Detail:
Type:  English Abstract; Journal Article    
Journal Detail:
Title:  Fa yi xue za zhi     Volume:  28     ISSN:  1004-5619     ISO Abbreviation:  Fa Yi Xue Za Zhi     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-12-06     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9426151     Medline TA:  Fa Yi Xue Za Zhi     Country:  China    
Other Details:
Languages:  chi     Pagination:  321-6     Citation Subset:  IM    
Affiliation:
Department of Forensic Medicine, Shanghai Medical College, Fudan University, Shanghai 200032, China. 11211010057@fudan.edu.cn
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