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Relation between Escherichia coli endonucleases specific for apurinic sites in DNA and exonuclease III.
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MedLine Citation:
PMID:  333397     Owner:  NLM     Status:  MEDLINE    
Contradictory data have recently been published from two different laboratories on the presence vs absence of an intrinsic endonucliolytic activity of E. coli exonuclease III at apurinic sites in double-stranded DNA. It is shown here that an endonuclease activity of this specificity co-chromatographs exactly with exonuclease III on phosphocellulose and Sephadex G-75 columns, indicating that the endonuclease and exonuclease activities are due to the same enzyme. In addition, another E. coli endonuclease specific for apurinic sites exists, which can be separated from exonuclease III by the same chromatographic procedures.
S Ljungquist; T Lindahl
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Nucleic acids research     Volume:  4     ISSN:  0305-1048     ISO Abbreviation:  Nucleic Acids Res.     Publication Date:  1977 Aug 
Date Detail:
Created Date:  1977-11-30     Completed Date:  1977-11-30     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0411011     Medline TA:  Nucleic Acids Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  2871-9     Citation Subset:  IM    
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MeSH Terms
Apurinic Acid*
Deoxyribonucleases / isolation & purification,  metabolism*
Endonucleases / isolation & purification,  metabolism*
Escherichia coli / enzymology*
Exonucleases / isolation & purification,  metabolism*
Substrate Specificity
Reg. No./Substance:
0/Polynucleotides; 11002-22-5/Apurinic Acid; 9007-49-2/DNA; EC 3.1.-/Deoxyribonucleases; EC 3.1.-/Endonucleases; EC 3.1.-/Exonucleases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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Journal Information
Journal ID (nlm-ta): Nucleic Acids Res
ISSN: 0305-1048
ISSN: 1362-4962
Article Information
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Print publication date: Month: 8 Year: 1977
Volume: 4 Issue: 8
First Page: 2871 Last Page: 2879
ID: 342615
PubMed Id: 333397

Relation between Escherichia coli endonucleases specific for apurinic sites in DNA and exonuclease III.
S Ljungquist
T Lindahl

Article Categories:
  • Research Article

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