Document Detail

Relation among neuronal death, cell proliferation and neuronal differentiation in the gerbil main olfactory bulb after transient cerebral ischemia.
MedLine Citation:
PMID:  20405201     Owner:  NLM     Status:  MEDLINE    
Neurogenesis occurs during the embryonic stage and throughout life. Brain injuries such as ischemic insults enhance cell proliferation in some areas of the brain. We examined proliferation of newly generated cells in each layer of the gerbil main olfactory bulb (MOB) after 5 min of transient cerebral ischemia using bromodeoxyuridine (BrdU) immunohistochemistry. Ischemia-related neuronal death in the MOB was not detected using Fluoro-Jade B histofluorescence and TUNEL staining. Many BrdU-positive ((+)) cells were found in the rostral migratory stream in control and ischemic MOBs. Significant increase of BrdU(+) cells was observed in the granule cell layer (GCL) and glomerular layer (GL) from 15 days post-ischemia, and BrdU(+) cells were very much higher than those of the control group 30 days post-ischemia. At this time point after ischemia/reperfusion, a few BrdU(+) cells in the GL and GCL were co-localized with calretinin(+) cells, and many BrdU(+) cells expressed doublecortin, a marker of immature neurons. These results indicate that cell proliferation is increased in the GCL and GL without apparent neuronal loss from 15 days after transient cerebral ischemia in gerbils.
Jung Hoon Choi; Ki-Yeon Yoo; Ok Kyu Park; Choong Hyun Lee; Sung Koo Kim; In Koo Hwang; Yun Lyul Lee; Hyung-Cheul Shin; Moo-Ho Won
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-04-20
Journal Detail:
Title:  Cellular and molecular neurobiology     Volume:  30     ISSN:  1573-6830     ISO Abbreviation:  Cell. Mol. Neurobiol.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-08-02     Completed Date:  2011-01-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8200709     Medline TA:  Cell Mol Neurobiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  929-38     Citation Subset:  IM    
Department of Anatomy and Neurobiology, and Institute of Neurodegeneration and Neuroregeneration, College of Medicine, Hallym University, Chuncheon, 200-702, South Korea.
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MeSH Terms
Blotting, Western
Bromodeoxyuridine / metabolism
Calcium-Binding Protein, Vitamin D-Dependent / metabolism
Cell Death
Cell Differentiation*
Cell Proliferation
Fluorescent Antibody Technique
Gerbillinae / metabolism*
In Situ Nick-End Labeling
Ischemic Attack, Transient / metabolism,  pathology*
Microtubule-Associated Proteins / metabolism
Nerve Tissue Proteins / metabolism
Neurons / metabolism,  pathology*
Neuropeptides / metabolism
Olfactory Bulb / metabolism,  pathology*
Reg. No./Substance:
0/Calcium-Binding Protein, Vitamin D-Dependent; 0/Microtubule-Associated Proteins; 0/Nerve Tissue Proteins; 0/Neuropeptides; 0/calretinin; 0/doublecortin protein; 59-14-3/Bromodeoxyuridine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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