Document Detail

Relatedness of cytotoxins from geographically diverse isolates of Moraxella bovis.
MedLine Citation:
PMID:  17540518     Owner:  NLM     Status:  MEDLINE    
To determine whether amino acid sequence variation exists in the Moraxella bovis (M. bovis) cytotoxin (MbxA) from geographically diverse M. bovis isolated in the United States, mbxA was amplified and sequenced. The MbxA deduced amino acid sequence from M. bovis originally isolated in California, Washington, North Carolina, and Georgia, as well as reference strains of M. bovis isolated at the National Animal Disease Laboratory, Ames, IA, USA, all encoded a nearly identical 927 amino acid protein. MbxA from two of the four California isolates (SFS 9a and SFS 100a) differed from all other isolates at two sites at which the polar amino acids glutamine (position 666) and asparagine (position 823) were replaced by ionized amino acids glutamic acid and aspartic acid, respectively. Rabbit antiserum to the expressed carboxy terminus (amino acids 590-927) of MbxA from M. bovis (Tifton I) neutralized the hemolytic activity of SFS 9a and SFS 100a. The M. bovis cytotoxin appears to be conserved amongst geographically diverse isolates of M. bovis from the USA. Antiserum against the carboxy terminus of MbxA common to the majority of isolates neutralized the hemolytic activity of two strains with a divergent MbxA deduced amino acid sequence. Vaccines against IBK that incorporate MbxA as antigen may offer protection against geographically diverse strains of M. bovis.
John A Angelos; Louise M Ball
Related Documents :
17208488 - Mycobacterium smegmatis produces an hbha homologue which is not involved in epithelial ...
23452788 - Battery-triggered ultrasensitive electrochemiluminescence detection on microfluidic pap...
22805178 - Flo11 expression and lipid biosynthesis are required for air liquid biofilm formation i...
24084938 - Enhanced lymphatic transport of bioactive lipids: cell culture study of polymethoxyflav...
10788438 - Proton re-uptake partitioning between uncoupling protein and atp synthase during benzoh...
2675638 - Amino acid transport systems in intestinal brush-border membranes from lepidopteran lar...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-05-03
Journal Detail:
Title:  Veterinary microbiology     Volume:  124     ISSN:  0378-1135     ISO Abbreviation:  Vet. Microbiol.     Publication Date:  2007 Oct 
Date Detail:
Created Date:  2007-08-27     Completed Date:  2008-01-15     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7705469     Medline TA:  Vet Microbiol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  382-6     Citation Subset:  IM    
Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, 2108 Tupper Hall, Davis, CA 95616, USA.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/EF436233;  EF436234;  EF436235;  EF436236;  EF436237;  EF436238;  EF436239;  EF436240;  EF436241;  EF436242;  EF436243
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Amino Acid Sequence
Base Sequence
Cattle Diseases / microbiology*
Cytotoxins / chemistry,  genetics*
DNA, Bacterial / chemistry,  genetics
Gene Amplification
Genetic Variation*
Keratoconjunctivitis, Infectious / microbiology*
Molecular Sequence Data
Moraxella (Moraxella) bovis / genetics,  metabolism*
Neutralization Tests / veterinary
Sequence Alignment
United States
Reg. No./Substance:
0/Cytotoxins; 0/DNA, Bacterial

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Campylobacter-induced interleukin-8 responses in human intestinal epithelial cells and primary intes...
Next Document:  Analytical applications of Fourier transform-infrared (FT-IR) spectroscopy in microbiology and prion...