Document Detail


Relapsing-remitting multiple sclerosis and human herpesvirus 6 active infection.
MedLine Citation:
PMID:  15477505     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Recent studies have focused on the relationship between human herpesvirus 6 (HHV-6) and multiple sclerosis (MS). OBJECTIVE: To analyze HHV-6 messenger RNA expression in patients with relapsing-remitting (RR) MS vs healthy blood donors (HBDs). DESIGN: One hundred fifty-four subjects were enrolled in the study: 105 patients with RRMS (32 in relapse) and 49 HBDs. Total DNA and messenger RNA were extracted from serum and blood samples, respectively, and analyzed by quantitative real-time reverse transcription-polymerase chain reaction for the detection of 3 HHV-6 immediate-early genes (U16/U17,U89/U90, and U94) and both HHV-6 variants (HHV-6A and HHV-6B). RESULTS: Active HHV-6 infection was detected in 16% of patients with RRMS vs 0% of HBDs (P = .003). Seven patients with RRMS with exacerbation had HHV-6 active replication, and the virus remained latent in only 1 of them. We did not find any statistically significant difference between HHV-6 active or latent infection for patients in remission (P = .12). Among patients with RRMS with HHV-6 active replication, viral load was higher when they experienced an acute attack than when in remission (P = .04). In those patients with RRMS who had an active infection only, HHV-6A was found. Cell-free HHV-6 DNA detected in serum samples confirmed the results. CONCLUSIONS: The results show that a subset of patients with RRMS experience HHV-6 active infection, and there likely is an association between the viral active replication and relapses; therefore, HHV-6 active infection may imply a greater risk of exacerbations in a subgroup of patients with RRMS.
Authors:
Roberto Alvarez-Lafuente; Virginia De las Heras; Manuel Bartolomé; Juan José Picazo; Rafael Arroyo
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Archives of neurology     Volume:  61     ISSN:  0003-9942     ISO Abbreviation:  Arch. Neurol.     Publication Date:  2004 Oct 
Date Detail:
Created Date:  2004-10-12     Completed Date:  2004-12-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372436     Medline TA:  Arch Neurol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1523-7     Citation Subset:  AIM; IM    
Affiliation:
Servicio de Neurología, Hospital Clínico San Carlos, 28040 Madrid, Spain. labesmul@hcsc.es
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MeSH Terms
Descriptor/Qualifier:
Adult
DNA, Viral / blood
Female
Herpesviridae Infections / blood,  complications,  virology*
Herpesvirus 6, Human / genetics,  isolation & purification*
Humans
Male
Middle Aged
Multiple Sclerosis, Relapsing-Remitting / blood,  complications,  virology*
RNA, Messenger / biosynthesis
Reverse Transcriptase Polymerase Chain Reaction / methods
Viral Load / statistics & numerical data
Chemical
Reg. No./Substance:
0/DNA, Viral; 0/RNA, Messenger
Comments/Corrections
Comment In:
Arch Neurol. 2004 Oct;61(10):1500-2   [PMID:  15477500 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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