Document Detail

Rejuvenation of periosteal chondrogenesis using local growth factor injection.
MedLine Citation:
PMID:  19064326     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To examine the potential for rejuvenation of aged periosteum by local injection of transforming growth factor-beta1 (TGF-beta1) and insulin-like growth factor-1 (IGF-1) alone or in combination to induce cambium cell proliferation and enhance in vitro periosteal cartilage formation. METHODS: A total of 367 New Zealand white rabbits (6, 12, and 24+ month-old) received subperiosteal injections of TGF-beta1 and/or IGF-1 percutaneously. After 1, 3, 5, or 7 days, the rabbits were sacrificed and cambium cellularity or in vitro cartilage forming capacity was determined. RESULTS: A significant increase in cambium cellularity and thickness, and in vitro cartilage formation was observed after injection of TGF-beta1 alone or in combination with IGF-1. In 12 month-old rabbits, mean cambium cellularity increased 5-fold from 49 to 237 cells/mm and in vitro cartilage production increased 12-fold from 0.8 to 9.7 mg 7 days after TGF-beta1 (200 ng) injection compared to vehicle controls (P<0.0001). A correlation was observed between cambium cellularity and in vitro cartilage production (R2=0.98). An added benefit of IGF-1 plus TGF-beta1 on in vitro cartilage production compared to TGF-beta1 alone was observed in the 2 year-old rabbits. IGF-1 alone generally had no effect on either cambium cellularity or in vitro cartilage production in any of the age groups. CONCLUSIONS: These results clearly demonstrate that it is possible to increase cambium cellularity and in vitro cartilage production in aged rabbit periosteum, to levels comparable to younger rabbits, using local injection of TGF-beta1 alone or in combination with IGF-1, thereby rejuvenating aged periosteum.
G G Reinholz; J S Fitzsimmons; M E Casper; T J Ruesink; H W Chung; J C Schagemann; S W O'Driscoll
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2008-11-06
Journal Detail:
Title:  Osteoarthritis and cartilage / OARS, Osteoarthritis Research Society     Volume:  17     ISSN:  1522-9653     ISO Abbreviation:  Osteoarthr. Cartil.     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-05-20     Completed Date:  2010-02-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9305697     Medline TA:  Osteoarthritis Cartilage     Country:  England    
Other Details:
Languages:  eng     Pagination:  723-34     Citation Subset:  IM    
Department of Orthopedic Surgery, Cartilage and Connective Tissue Research Laboratory, Mayo Clinic, 200 First St SW, Rochester, MN 55905, USA.
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MeSH Terms
Cartilage, Articular / drug effects*,  physiopathology
Cell Proliferation / drug effects*
Chondrogenesis / drug effects
Insulin-Like Growth Factor I / administration & dosage*
Periosteum / drug effects*,  physiopathology
Rejuvenation / physiology*
Transforming Growth Factor beta1 / administration & dosage*
Grant Support
Reg. No./Substance:
0/Transforming Growth Factor beta1; 67763-96-6/Insulin-Like Growth Factor I

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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