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Rejuvenation of Nucleus Pulposus Cells Using Extracellular Matrix Deposited by Synovium-Derived Stem Cells.
MedLine Citation:
PMID:  21540772     Owner:  NLM     Status:  Publisher    
ABSTRACT: Study Design. After plating for six passages on either plastic flasks or extracellular matrix (ECM) deposited by synovium-derived stem cells (SDSCs), expanded nucleus pulposus (NP) cells were evaluated for redifferentiation capacity.Objective. The aim was to assess the feasibility of using ECM deposited by a tissue-specific stem cell to provide a 3D microenvironment for NP cell rejuvenation.Summary of Background Data. Autologous disc cell-based therapy is a promising approach for intervertebral disc (IVD) regeneration. Unfortunately, the current in vitro expansion of NP cells in monolayer results in dedifferentiation of these cells.Methods. Primary NP cells were plated on either plastic flasks or ECM for six consecutive passages. At each passage, cell numbers were counted for proliferation rate, cell phenotype was evaluated using flow cytometry, and cell differentiation status was assessed using real-time PCR. The pellets from expanded NP cells at passages 1, 4, and 6 were incubated in a serum-free defined medium for 14 days. Redifferentiation capacity of the expanded NP cells was evaluated using histology, biochemistry, and real-time PCR.Results. NP cells expanded on ECM grew much faster with a smaller size and fibroblast-like shape compared to those on plastic flasks. ECM-treated NP cells acquired an enhanced CD90 expression and higher mRNA levels of types I, II, and X collagen and aggrecan, as well as a robust redifferentiation capacity, evidenced by dramatically increased type II collagen, aggrecan, and Sox9 and decreased type I collagen for up to six passages.Conclusion. SDSC-derived ECM can provide a tissue-specific microenvironment for the rejuvenation of NP cells with a higher proliferation rate and redifferentiation capacity. These characteristics may play a role in improving an autologous disc cell-based minimally invasive therapeutic approach toward physiological reconstruction of a biologically functional disc in the clinical setting.
Fan He; Ming Pei
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-5-2
Journal Detail:
Title:  Spine     Volume:  -     ISSN:  1528-1159     ISO Abbreviation:  -     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-5-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7610646     Medline TA:  Spine (Phila Pa 1976)     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
From the Stem Cell and Tissue Engineering Laboratory, Department of Orthopaedics, and Exercise Physiology, West Virginia University, Morgantown, WV 26506.
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