Document Detail


Rehydration of air-dried cervical smears: a feasible alternative to conventional wet fixation.
MedLine Citation:
PMID:  14551006     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To evaluate the effect of rehydration of air-dried cervical smears on staining quality and to find out whether rehydration techniques can be adopted as an alternative method, especially in high-volume, resource-limited settings. METHODS: A total of 950 paired wet-fixed and air-dried-rehydrated cervical smears collected by paramedical workers from an urban slum of Delhi, India, were compared for staining quality by assessing different cytoplasmic and nuclear parameters. RESULTS: The staining quality in air-dried-rehydrated smears was satisfactory and comparable to wet-fixed smears (61.3% versus 63.6%). The staining was superior in rehydrated smears in 26.5%, as compared with 15.4% in wet-fixed ones (P <.001). Unsatisfactory staining was observed in 12.2% of rehydrated smears, as compared with 21% of wet-fixed smears. The smear background was cleaner in rehydrated smears. Red blood cells were observed in only 3% of rehydrated smears, in comparison to 12% in wet-fixed ones (P <.001). CONCLUSION: Rehydration of air-dried cervical smears is a suitable alternative to wet-fixed smears. The staining quality is either the same as or better than wet-fixed smears, and the unsatisfactory rate is lower. This technique is simple and can be conveniently adopted in resource-limited settings.
Authors:
Sanjay Gupta; Pushpa Sodhani; Krishan Lal Chachra
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Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article    
Journal Detail:
Title:  Obstetrics and gynecology     Volume:  102     ISSN:  0029-7844     ISO Abbreviation:  Obstet Gynecol     Publication Date:  2003 Oct 
Date Detail:
Created Date:  2003-10-10     Completed Date:  2003-11-04     Revised Date:  2009-10-26    
Medline Journal Info:
Nlm Unique ID:  0401101     Medline TA:  Obstet Gynecol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  761-4     Citation Subset:  AIM; IM    
Affiliation:
Division of Cytopathology, Institute of Cytology and Preventive Oncology, New Delhi, India. sanjaydr17@hotmail.com
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Aged
Cervical Intraepithelial Neoplasia / diagnosis*
Cervix Uteri / cytology
Female
Humans
Middle Aged
Predictive Value of Tests
Specimen Handling / methods
Staining and Labeling / methods
Uterine Cervical Neoplasms / diagnosis*
Vaginal Smears / methods,  standards*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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