| Rehydration of air-dried cervical smears: a feasible alternative to conventional wet fixation. | |
| | |
MedLine Citation:
|
PMID: 14551006 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
OBJECTIVE: To evaluate the effect of rehydration of air-dried cervical smears on staining quality and to find out whether rehydration techniques can be adopted as an alternative method, especially in high-volume, resource-limited settings. METHODS: A total of 950 paired wet-fixed and air-dried-rehydrated cervical smears collected by paramedical workers from an urban slum of Delhi, India, were compared for staining quality by assessing different cytoplasmic and nuclear parameters. RESULTS: The staining quality in air-dried-rehydrated smears was satisfactory and comparable to wet-fixed smears (61.3% versus 63.6%). The staining was superior in rehydrated smears in 26.5%, as compared with 15.4% in wet-fixed ones (P <.001). Unsatisfactory staining was observed in 12.2% of rehydrated smears, as compared with 21% of wet-fixed smears. The smear background was cleaner in rehydrated smears. Red blood cells were observed in only 3% of rehydrated smears, in comparison to 12% in wet-fixed ones (P <.001). CONCLUSION: Rehydration of air-dried cervical smears is a suitable alternative to wet-fixed smears. The staining quality is either the same as or better than wet-fixed smears, and the unsatisfactory rate is lower. This technique is simple and can be conveniently adopted in resource-limited settings. |
| | |
Authors:
|
Sanjay Gupta; Pushpa Sodhani; Krishan Lal Chachra |
Related Documents
:
|
9404706 - Intensified therapy for infants with acute lymphoblastic leukemia: results from the dan... 22408586 - Assessment of indoor air pollution in homes with infants. 186236 - The aetiology of diarrhoea in newborn infants. 2842406 - Seroconversion patterns to four human rotavirus serotypes in hospitalized infants with ... 10636596 - Limb anomalies among offspring of women with a septate uterus: a report of three cases. 15038906 - Routine examination of the newborn: the emren study. evaluation of an extension of the ... |
Publication Detail:
|
Type: Comparative Study; Evaluation Studies; Journal Article |
Journal Detail:
|
Title: Obstetrics and gynecology Volume: 102 ISSN: 0029-7844 ISO Abbreviation: Obstet Gynecol Publication Date: 2003 Oct |
Date Detail:
|
Created Date: 2003-10-10 Completed Date: 2003-11-04 Revised Date: 2009-10-26 |
Medline Journal Info:
|
Nlm Unique ID: 0401101 Medline TA: Obstet Gynecol Country: United States |
Other Details:
|
Languages: eng Pagination: 761-4 Citation Subset: AIM; IM |
Affiliation:
|
Division of Cytopathology, Institute of Cytology and Preventive Oncology, New Delhi, India. sanjaydr17@hotmail.com |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adolescent Adult Aged Cervical Intraepithelial Neoplasia / diagnosis* Cervix Uteri / cytology Female Humans Middle Aged Predictive Value of Tests Specimen Handling / methods Staining and Labeling / methods Uterine Cervical Neoplasms / diagnosis* Vaginal Smears / methods, standards* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Prenatal screening for infectious diseases and opportunities for prevention.
Next Document: Changes in cervical cancer incidence after three decades of screening US women less than 30 years ol...