Document Detail

Regulatory volume increase (RVI) by in situ and isolated bovine articular chondrocytes.
MedLine Citation:
PMID:  16897756     Owner:  NLM     Status:  MEDLINE    
Metabolism of the matrix by chondrocytes is sensitive to alterations in cell volume that occur, for example, during static loading and osteoarthritis. The ability of chondrocytes to respond to changes in volume could be important, and this study was aimed at testing the hypothesis that chondrocytes can regulate their volume following cell shrinking by regulatory volume increase (RVI). We used single cell fluorescence imaging of in situ bovine articular chondrocytes, cells freshly isolated into 280 or 380 mOsm, or 2-D cultured chondrocytes loaded with calcein or fura-2, to investigate RVI and changes to [Ca2+]i during shrinkage. Following a 42% hyperosmotic challenge, chondrocytes rapidly shrunk, however, only approximately 6% of the in situ or freshly isolated chondrocytes demonstrated RVI. This contrasted with 2D-cultured chondrocytes where approximately 54% of the cells exhibited RVI. The rate of RVI was the same for all preparations. During the 'post-RVD/RVI protocol', approximately 60% of the in situ and freshly isolated chondrocytes demonstrated RVD, but only approximately 5% showed RVI. There was no relationship between [Ca2+]i and RVI either during hyperosmotic challenge, or during RVD suggesting that changes to [Ca2+]i were not required for RVI. Depolymerisation of the actin cytoskeleton by latrunculin, increased RVI by freshly isolated chondrocytes, in a bumetanide-sensitive manner. The results showed that in situ and freshly isolated articular chondrocytes have only limited RVI capacity. However, RVI was stimulated by treating freshly isolated chondrocytes with latrunculin B and following 2D culture of chondrocytes, suggesting that cytoskeletal integrity plays a role in regulating RVI activity which appears to be mediated principally by the Na+ - K+ -2Cl- cotransporter.
Mark J P Kerrigan; Corinne S V Hook; Ala' Qusous; Andrew C Hall
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cellular physiology     Volume:  209     ISSN:  0021-9541     ISO Abbreviation:  J. Cell. Physiol.     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2006-09-04     Completed Date:  2006-10-26     Revised Date:  2007-08-13    
Medline Journal Info:
Nlm Unique ID:  0050222     Medline TA:  J Cell Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  481-92     Citation Subset:  IM    
Copyright Information:
Copyright 2006 Wiley-Liss, Inc.
Department of Human and Health Sciences, School of Biosciences, University of Westminster, London, UK.
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MeSH Terms
Bumetanide / pharmacology
Cartilage, Articular / cytology*
Cell Separation
Cell Size* / drug effects
Cells, Cultured
Chondrocytes / cytology*
Fluoresceins / metabolism
Fura-2 / metabolism
Osmotic Pressure / drug effects
Grant Support
075753/Z/04/Z//Wellcome Trust; VS/05/WEST/A2//Wellcome Trust
Reg. No./Substance:
0/Fluoresceins; 1461-15-0/fluorexon; 28395-03-1/Bumetanide; 96314-98-6/Fura-2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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