Document Detail


Regulatory volume decrease in human esophageal epithelial cells.
MedLine Citation:
PMID:  12223353     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In vivo human esophageal epithelial cells are regularly exposed to hyposmolal stress. This stress, however, only becomes destructive when the surface epithelial cell (barrier) layers are breached and there is contact of the hyposmolal solution with the basolateral cell membranes. The present investigation was designed to examine the effects of hyposmolal stress in the latter circumstance using as a model for human esophageal epithelial cells the noncancer-derived HET-1A cell line. Cell volume and the response to hyposmolal stress in suspensions of HET-1A cells were determined by cell passage through a Coulter Counter Multisizer II. HET-1A cells behaved as osmometers over the range of 280 to 118 mosmol/kg H(2)O with rapid increases in cell volume < or = 15-20% above baseline. Following swelling, the cells exhibited regulatory volume decrease (RVD), restoring baseline volume within 30 min, despite continued hyposmolal stress. With the use of pharmacologic agents and ion substitutions, RVD appeared to result from rapid activation of parallel K(+) and Cl(-) conductance pathways and this was subsequently joined by activation of a KCl cotransporter. Exposure to hyposmolal stress in an acidic environment, pH 6.6, inhibited, but did not abolish, RVD. These data indicate that human esophageal epithelial cells can protect against hyposmolal stress by RVD and that the redundancy in mechanisms may, to some extent, serve as added protection in patients with reflux disease when hyposmolal stress may occur in an acidic environment.
Authors:
Geraldine S Orlando; Nelia A Tobey; Paul Wang; Solange Abdulnour-Nakhoul; Roy C Orlando
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  American journal of physiology. Gastrointestinal and liver physiology     Volume:  283     ISSN:  0193-1857     ISO Abbreviation:  Am. J. Physiol. Gastrointest. Liver Physiol.     Publication Date:  2002 Oct 
Date Detail:
Created Date:  2002-09-11     Completed Date:  2002-10-16     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  100901227     Medline TA:  Am J Physiol Gastrointest Liver Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  G932-7     Citation Subset:  IM    
Affiliation:
Department of Medicine, Tulane University School of Medicine and Veterans Administration Medical Center, New Orleans, Louisiana 70112 - 2699, USA. gorlando@tulane.edu
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MeSH Terms
Descriptor/Qualifier:
4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / analogs & derivatives*,  pharmacology
Cell Line, Transformed
Cell Size
Chloride Channels / antagonists & inhibitors
Chlorides / metabolism
Electric Conductivity
Epithelial Cells / cytology
Esophagus / cytology*
Glycolates / pharmacology
Humans
Hydrogen-Ion Concentration
Hypotonic Solutions
Osmolar Concentration
Potassium / metabolism
Potassium Channel Blockers
Symporters
Grant Support
ID/Acronym/Agency:
DK-36013/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Chloride Channels; 0/Chlorides; 0/Glycolates; 0/Hypotonic Solutions; 0/Potassium Channel Blockers; 0/Symporters; 0/potassium-chloride symporters; 53005-05-3/4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; 54197-05-6/MK 473; 61481-03-6/dihydro-DIDS; 7440-09-7/Potassium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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