Document Detail

Regulatory volume decrease of cultured human fibroblasts involves changes in intracellular amino-acid pool.
MedLine Citation:
PMID:  8312358     Owner:  NLM     Status:  MEDLINE    
Regulatory volume decrease (RVD) has been studied in cultured human fibroblasts incubated in a complete growth medium at low osmolality (215 mosmolal). After the initial swelling induced by hypotonic treatment, cells recover their volume almost completely within about 60 min. This RVD is associated with comparable losses of cell potassium and amino acids. After an initial increase, cell content of sodium is kept at values close to control. Chromatographic analysis of intracellular amino-acid pool has shown that RVD-associated decrease in cell amino acids is due for the most part to changes in the intracellular concentration of L-glutamine. RVD-exerting cells undergo a rapid and marked depolarization that is maintained after cell volume recovery. This change in membrane potential has been detected with measurements of both the transmembrane distribution ratios of L-arginine and of fluorescence of potential-sensitive dye bis-oxonol. Due to depolarization, the trans-membrane gradient of sodium electrochemical potential is lowered. It is proposed that cell depolarization concurs to keep the intracellular concentration of amino acids low by inhibiting sodium-coupled uptake through system A.
V Dall'Asta; P A Rossi; O Bussolati; G C Gazzola
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1220     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1994 Jan 
Date Detail:
Created Date:  1994-03-21     Completed Date:  1994-03-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  139-45     Citation Subset:  IM    
Istituto di Patologia Generale, Università degli Studi di Parma, Italy.
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MeSH Terms
Amino Acids / analysis*
Cations / analysis
Cell Size*
Cells, Cultured
Hypotonic Solutions
Membrane Potentials
Reg. No./Substance:
0/Amino Acids; 0/Cations; 0/Hypotonic Solutions

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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