Document Detail


Regulatory volume decrease is actively modulated during the cell cycle.
MedLine Citation:
PMID:  12209886     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Nasopharyngeal carcinoma cells, CNE-2Z, when swollen by 47% hypotonic solution, exhibited a regulatory volume decrease (RVD). The RVD was inhibited by extracellular applications of the chloride channel blockers tamoxifen (30 microM; 61% inhibition), 5-nitro-2-(3-phenylpropylamino)benzoic acid (NPPB, 100 microM; 60% inhibition), and ATP (10 mM; 91% inhibition). The level and time constant of RVD varied greatly between cells. Most cells conducted an incomplete RVD, but a few had the ability to recover their volume completely. There was no obvious correlation between cell volume and RVD capacity. Flow cytometric analysis showed that highly synchronous cells were obtained by the mitotic shake-off technique and that the cells progressed through the cell cycle synchronously when incubated in culture medium. Combined application of DNA synthesis inhibitors, thymidine and hydroxyurea arrested cells at the G1/S boundary and 87% of the cells reached S phase 4 h after being released. RVD capacity changed significantly during the cell cycle progression in cells synchronized by shake-off technique. RVD capacity being at its highest in G1 phase and lowest in S phase. The RVD capacity in G1 (shake-off cells sampled after 4 h of incubation), S (obtained by chemical arrest), and M cells (selected under microscope) was 73, 33, and 58%, respectively, and the time constants were 435, 769, and 2,000 sec, respectively. We conclude that RVD capacity is actively modulated in the cell cycle and RVD may play an important role in cell cycle progress.
Authors:
Liwei Wang; Lixin Chen; Linyan Zhu; Michelle Rawle; Sihuai Nie; Jin Zhang; Zhong Ping; Cai Kangrong; Tim J C Jacob
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cellular physiology     Volume:  193     ISSN:  0021-9541     ISO Abbreviation:  J. Cell. Physiol.     Publication Date:  2002 Oct 
Date Detail:
Created Date:  2002-09-04     Completed Date:  2002-09-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0050222     Medline TA:  J Cell Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  110-9     Citation Subset:  IM    
Copyright Information:
Copyright 2002 Wiley-Liss, Inc.
Affiliation:
School of Biosciences, Cardiff University, Cardiff, United Kingdom.
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphate / pharmacology
Carcinoma / drug therapy,  pathology,  physiopathology*
Cell Cycle / drug effects,  physiology
Cell Size / drug effects,  physiology
Chloride Channels / antagonists & inhibitors
Flow Cytometry
G1 Phase / drug effects
Humans
Hydroxyurea / pharmacology
Hypotonic Solutions / pharmacology
Mitosis / drug effects
Nasopharyngeal Neoplasms / drug therapy,  pathology,  physiopathology*
Nitrobenzoates / pharmacology
Nucleic Acid Synthesis Inhibitors / pharmacology
S Phase / drug effects
Tamoxifen / pharmacology
Thymidine / pharmacology
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Chloride Channels; 0/Hypotonic Solutions; 0/Nitrobenzoates; 0/Nucleic Acid Synthesis Inhibitors; 10540-29-1/Tamoxifen; 107254-86-4/5-nitro-2-(3-phenylpropylamino)benzoic acid; 127-07-1/Hydroxyurea; 50-89-5/Thymidine; 56-65-5/Adenosine Triphosphate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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