Document Detail


Regulatory molecules for coronary expressions of VEGF and its angiogenic receptor KDR in hypoestrogenic middle-aged female rats.
MedLine Citation:
PMID:  15124924     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We studied the effects of estrogen deprivation and replacement on the protein and gene expression levels molecules that can be considered to be essential for coronary angiogenesis in middle-aged female rats. The animals were subjected to sham operation, ovariectomy, or ovariectomy with estrogen replacement therapy (ERT). Following ovariectomy, protein and gene expressions of vascular endothelial growth factor (VEGF) and its angiogenic receptor (KDR) showed a marked decline in coronary vessels, as determined by immunohistochemistry and in situ hybridization. ERT resulted in restoration of the ovariectomy-induced changes to intact levels. The coronary expression level of basic fibroblast growth factor was unaffected by estrogen deprivation or treatment. The changes in VEGF and KDR expressions were strongly associated with those in endothelial nitric oxide synthase (eNOS) expression in coronary vessels. Moreover, the age- and gender-dependent accumulation of hypoxia-inducible factor-1alpha (HIF-1alpha) protein appeared to be a determinant molecule of VEGF expression in middle-aged female rats. We reached a conclusion that the VEGF-KDR system plays a key role in coronary angiogenesis in hypoestrogenic elderly women and is critically regulated by estrogen, eNOS and HIF-1alpha.
Authors:
Subrina Jesmin; Ichiro Sakuma; Yuichi Hattori; Akira Kitabatake
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular and cellular biochemistry     Volume:  259     ISSN:  0300-8177     ISO Abbreviation:  Mol. Cell. Biochem.     Publication Date:  2004 Apr 
Date Detail:
Created Date:  2004-05-04     Completed Date:  2005-01-25     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0364456     Medline TA:  Mol Cell Biochem     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  189-96     Citation Subset:  IM    
Affiliation:
Department of Cardiovascular Medicine, Hokkaido University Graduate School of Medicine, Sapporo, Japan.
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MeSH Terms
Descriptor/Qualifier:
Age Factors
Animals
Coronary Vessels / metabolism*
Estradiol / administration & dosage*
Estrogens / metabolism
Female
Gene Expression Regulation / drug effects
Hypoxia-Inducible Factor 1, alpha Subunit
Immunohistochemistry
In Situ Hybridization
Neovascularization, Physiologic / drug effects
Nitric Oxide Synthase / metabolism
Nitric Oxide Synthase Type III
Ovariectomy
Rats
Rats, Inbred WF
Sex Factors
Transcription Factors / metabolism
Vascular Endothelial Growth Factor A / metabolism*
Vascular Endothelial Growth Factor Receptor-2 / metabolism*
Chemical
Reg. No./Substance:
0/Estrogens; 0/Hypoxia-Inducible Factor 1, alpha Subunit; 0/Transcription Factors; 0/Vascular Endothelial Growth Factor A; 50-28-2/Estradiol; EC 1.14.13.39/Nitric Oxide Synthase; EC 1.14.13.39/Nitric Oxide Synthase Type III; EC 1.14.13.39/Nos3 protein, rat; EC 2.7.10.1/Vascular Endothelial Growth Factor Receptor-2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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