Document Detail


Regulatory mechanisms underlying agmatine homeostasis in humans.
MedLine Citation:
PMID:  18832451     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Regulation of agmatine homeostasis has so far only been poorly defined. In the present study, three mechanisms regulating human agmatine homeostasis were investigated. 1) Enzymatic regulation: expression of arginine decarboxylase, diamine oxidase, and ornithine decarboxylase in human colon neoplastic tissue was, at the mRNA level, about 75% and 50% lower and 150% higher, respectively, than in the adjacent normal tissue; expression of agmatinase was unchanged. 2) Bacteria-derived agmatine: ten representative bacteria strains of the human intestinal microbiota considerably differed in agmatine production and its efflux into their surrounding fluid, suggesting that the composition of the intestinal microbiota influences the agmatine availability in the gut lumen for absorption. 3) Regulation of blood plasma agmatine concentration by the human liver: at low concentrations in portal venous blood plasma, agmatine either slightly increased or further decreased in blood plasma through liver passage. Above a threshold of 14 ng/ml agmatine in the portal venous blood plasma, substantial hepatic agmatine removal from blood occurred. Taken together, a perturbation of agmatine homeostasis has been proven to be involved in the regulation of malignant cell proliferation. The amount of agmatine available for absorption, which is an important physiological source of agmatine in the human organism, should differ considerably depending on the composition of the bacterial flora in the chyme since the various species of intestinal bacteria largely differ in their ability to form agmatine. Finally, evidence has been presented that the liver plays a crucial physiological role in the maintenance of agmatine homeostasis in the human organism.
Authors:
Britta Haenisch; Ivar von Kügelgen; Heinz Bönisch; Manfred Göthert; Tilman Sauerbruch; Michael Schepke; Günter Marklein; Katja Höfling; Detlev Schröder; Gerhard J Molderings
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-10-02
Journal Detail:
Title:  American journal of physiology. Gastrointestinal and liver physiology     Volume:  295     ISSN:  0193-1857     ISO Abbreviation:  Am. J. Physiol. Gastrointest. Liver Physiol.     Publication Date:  2008 Nov 
Date Detail:
Created Date:  2008-11-06     Completed Date:  2009-01-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100901227     Medline TA:  Am J Physiol Gastrointest Liver Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  G1104-10     Citation Subset:  IM    
Affiliation:
Inst. of Human Genetics, Univ. Hospital of Bonn, D-53111 Bonn, Germany.
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MeSH Terms
Descriptor/Qualifier:
Aged
Aged, 80 and over
Agmatine / metabolism*
Bacteria / metabolism
Colon / microbiology*
Female
Homeostasis
Humans
Male
Middle Aged
Chemical
Reg. No./Substance:
306-60-5/Agmatine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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