Document Detail


Regulatory mechanisms in the luminal and portal release of vasoactive intestinal polypeptide during vagal nerve stimulation in the cat.
MedLine Citation:
PMID:  2907169     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effect of vagal stimulation in chloralose-anesthetized cats on release of vasoactive intestinal polypeptide into the jejunal lumen and portal venous blood was tested simultaneously, and the effect of atropine and hexamethonium was investigated to elucidate the regulatory mechanisms involved in the release. Vagal stimulation caused a significant increase in vasoactive intestinal polypeptide concentrations in the luminal perfusates. A significant concomitant increase was seen in portal plasma. Gel filtration chromatography of luminal and portal samples demonstrated that the vasoactive intestinal polypeptide coeluted with synthetic porcine vasoactive intestinal polypeptide. Vasoactive intestinal polypeptide infusion at 80 and 160 pmol/kg.min produced portal plasma levels of at least 3000 pM but did not increase vasoactive intestinal polypeptide concentrations in the luminal perfusates. Thus, luminal vasoactive intestinal polypeptide originates from gastrointestinal tissue rather than by transduction from the circulation. Vagally induced release of vasoactive intestinal polypeptide into the lumen and portal plasma was not abolished by atropine but was totally suppressed by hexamethonium. The regulatory mechanisms controlling the parallel release of vasoactive intestinal polypeptide into both the jejunal lumen and portal circulation are identical and involve a non-muscarinic process which is under cholinoceptive, nicotinic control.
Authors:
M A Blank; K Kimura; B M Jaffe
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Regulatory peptides     Volume:  23     ISSN:  0167-0115     ISO Abbreviation:  Regul. Pept.     Publication Date:  1988 Oct 
Date Detail:
Created Date:  1989-04-26     Completed Date:  1989-04-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8100479     Medline TA:  Regul Pept     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  15-26     Citation Subset:  IM    
Affiliation:
Department of Surgery, State University of New York Health Science Center, Brooklyn 11203.
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MeSH Terms
Descriptor/Qualifier:
Animals
Atropine / pharmacology
Cats
Electric Stimulation
Hexamethonium
Hexamethonium Compounds / pharmacology
Jejunum / drug effects,  physiology*
Kinetics
Male
Reference Values
Vagus Nerve / physiology*
Vasoactive Intestinal Peptide / blood,  secretion*
Chemical
Reg. No./Substance:
0/Hexamethonium Compounds; 37221-79-7/Vasoactive Intestinal Peptide; 51-55-8/Atropine; 60-26-4/Hexamethonium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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