| Regulatory mechanisms of cellular respiration. III. Enzyme distribution in the cell. Its influence on the metabolism of pyruvic acid by bakers' yeast. | |
| | |
| Jump to Full Text | |
MedLine Citation:
|
PMID: 14824492 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The rate of the aerobic metabolism of pyruvic acid by bakers' yeast cells is determined mainly by the amount of undissociated acid present. As a consequence, the greatest rate of oxidation was observed at pH 2.8. Oxidation, at a slow rate, started at pH 1.08; at pH 9.4 there was no oxidation at all. The anaerobic metabolism, only a fraction of the aerobic, was observed only in acid solutions. There was none at pH values higher than 3. Pyruvic acid in the presence of oxygen was oxidized directly to acetic acid; in the absence of oxygen it was metabolized mainly by dismutation to lactic and acetic acids, and CO(2). Acetic acid formation was demonstrated on oxidation of pyruvic acid at pH 1.91, and on addition of fluoroacetic acid. Succinic acid formation was shown by addition of malonic acid. These metabolic pathways in a cell so rich in carboxylase may be explained by the arrangement of enzymes within the cell, so that carboxylase is at the center, while pyruvic acid oxidase is located at the periphery. Succinic and citric acids were oxidized only in acid solutions up to pH 4. Malic and alpha-ketoglutaric acids were not oxidized, undoubtedly because of lack of penetration. |
| | |
Authors:
|
E S G BARRON; M I ARDAO; M HEARON |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: The Journal of general physiology Volume: 34 ISSN: 0022-1295 ISO Abbreviation: J. Gen. Physiol. Publication Date: 1950 Nov |
Date Detail:
|
Created Date: 1951-12-01 Completed Date: 2004-02-15 Revised Date: 2011-09-28 |
Medline Journal Info:
|
Nlm Unique ID: 2985110R Medline TA: J Gen Physiol Country: Not Available |
Other Details:
|
Languages: eng Pagination: 211-24 Citation Subset: OM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Enzymes* Pyruvic Acid* Respiration* Yeasts* |
| Chemical | |
Reg. No./Substance:
|
0/Enzymes; 127-17-3/Pyruvic Acid |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
|
Journal Information Journal ID (nlm-ta): J Gen Physiol ISSN: 0022-1295 ISSN: 1540-7748 Publisher: The Rockefeller University Press |
Article Information Download PDF  Copyright © Copyright, 1950, by The Rockefeller Institute for Medical Research Received Day: 23 Month: 6 Year: 1950 Print publication date: Day: 20 Month: 11 Year: 1950 Volume: 34 Issue: 2 First Page: 211 Last Page: 224 ID: 2147256 PubMed Id: 14824492 |
| REGULATORY MECHANISMS OF CELLULAR RESPIRATION : III. ENZYME DISTRIBUTION IN THE CELL. ITS INFLUENCE ON THE METABOLISM OF PYRUVIC ACID BY BAKERS' YEAST | |
| E. S. Guzman Barron | |
| Maria Isabel Ardao | |
| Marion Hearon | |
| From the Chemical Division, Department of Medicine of The University of Chicago, Chicago |
|
Article Categories:
|
|
Previous Document: Enzyme kinetics and the rate of biological processes.
Next Document: Phage formation in Staphylococcus muscae cultures. VII. Partial purification of the protein factor n...