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Regulatory mechanisms of cellular respiration. III. Enzyme distribution in the cell. Its influence on the metabolism of pyruvic acid by bakers' yeast.
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MedLine Citation:
PMID:  14824492     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The rate of the aerobic metabolism of pyruvic acid by bakers' yeast cells is determined mainly by the amount of undissociated acid present. As a consequence, the greatest rate of oxidation was observed at pH 2.8. Oxidation, at a slow rate, started at pH 1.08; at pH 9.4 there was no oxidation at all. The anaerobic metabolism, only a fraction of the aerobic, was observed only in acid solutions. There was none at pH values higher than 3. Pyruvic acid in the presence of oxygen was oxidized directly to acetic acid; in the absence of oxygen it was metabolized mainly by dismutation to lactic and acetic acids, and CO(2). Acetic acid formation was demonstrated on oxidation of pyruvic acid at pH 1.91, and on addition of fluoroacetic acid. Succinic acid formation was shown by addition of malonic acid. These metabolic pathways in a cell so rich in carboxylase may be explained by the arrangement of enzymes within the cell, so that carboxylase is at the center, while pyruvic acid oxidase is located at the periphery. Succinic and citric acids were oxidized only in acid solutions up to pH 4. Malic and alpha-ketoglutaric acids were not oxidized, undoubtedly because of lack of penetration.
Authors:
E S G BARRON; M I ARDAO; M HEARON
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of general physiology     Volume:  34     ISSN:  0022-1295     ISO Abbreviation:  J. Gen. Physiol.     Publication Date:  1950 Nov 
Date Detail:
Created Date:  1951-12-01     Completed Date:  2004-02-15     Revised Date:  2011-09-28    
Medline Journal Info:
Nlm Unique ID:  2985110R     Medline TA:  J Gen Physiol     Country:  Not Available    
Other Details:
Languages:  eng     Pagination:  211-24     Citation Subset:  OM    
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MeSH Terms
Descriptor/Qualifier:
Enzymes*
Pyruvic Acid*
Respiration*
Yeasts*
Chemical
Reg. No./Substance:
0/Enzymes; 127-17-3/Pyruvic Acid
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Gen Physiol
ISSN: 0022-1295
ISSN: 1540-7748
Publisher: The Rockefeller University Press
Article Information
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Copyright © Copyright, 1950, by The Rockefeller Institute for Medical Research
Received Day: 23 Month: 6 Year: 1950
Print publication date: Day: 20 Month: 11 Year: 1950
Volume: 34 Issue: 2
First Page: 211 Last Page: 224
ID: 2147256
PubMed Id: 14824492

REGULATORY MECHANISMS OF CELLULAR RESPIRATION : III. ENZYME DISTRIBUTION IN THE CELL. ITS INFLUENCE ON THE METABOLISM OF PYRUVIC ACID BY BAKERS' YEAST
E. S. Guzman Barron
Maria Isabel Ardao
Marion Hearon
From the Chemical Division, Department of Medicine of The University of Chicago, Chicago


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