| Regulatory effects of Spirulina complex polysaccharides on growth of murine RSV-M glioma cells through toll-like receptor 4. | |
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MedLine Citation:
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PMID: 23134155 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The present study is the first to describe the suppressive effect of Spirulina complex polysaccharides (CPS) on glioma growth by down-regulating angiogenesis via a Toll-like receptor (TLR) 4 signal. Murine RSV-M glioma cells were implanted subcutaneously (S.C.) in C3H/HeN mice and into TLR4 mutant C3H/HeJ mice. Treatment with either Spirulina CPS or Escherichia coli (E. coli) lipopolysaccharides (LPS) strongly suppressed RSV-M glioma cell growth in C3H/HeN mice but not C3H/HeJ. Glioma cells stimulated production of interleukin (IL)-17 in both tumor-bearing C3H/HeN and C3H/HeJ mice. Treatment of E. coli LPS induced much higher level of IL-17 production in tumor-bearing C3H/HeN mice compared to tumor-bearing C3H/HeJ mice. In C3H/HeN mice, treatment with Spirulina CPS but not with E. coli LPS suppressed growth of re-transplanted glioma, suggesting that Spirulina CPS enhances the immune response. Administration of anti-CD8 antibodies, anti-CD4 and anti-CD8 antibodies or anti-asialo GM1 antibodies enhanced the tumor growth, suggesting that T cells, and natural killer (NK) cells or macrophages were involved in the suppression of the tumor growth by Spirulina CPS. Although anti-interferon (IFN)-γ antibodies had no effect on glioma cell growth, anti-IL-17 antibodies administered four days after tumor transplantation suppressed growth similarly to treatment with Spirulina CPS. Lower level of angiogenesis was observed in gliomas from Spirulina CPS-treated mice compared to those from saline- or E. coli LPS-treated mice. These results suggest that Spirulina CPS antagonizes glioma cell growth by down-regulating angiogenesis, in part, by regulating IL-17 production in C3H/HeN mice. |
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Authors:
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Yu Kawanishi; Akira Tominaga; Hiromi Okuyama; Satoshi Fukuoka; Takahiro Taguchi; Yutaka Kusumoto; Toshio Yawata; Yasunori Fujimoto; Shiro Ono; Keiji Shimizu |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-11-8 |
Journal Detail:
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Title: Microbiology and immunology Volume: - ISSN: 1348-0421 ISO Abbreviation: Microbiol. Immunol. Publication Date: 2012 Nov |
Date Detail:
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Created Date: 2012-11-8 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7703966 Medline TA: Microbiol Immunol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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© 2012 The Societies and Wiley Publishing Asia Pty Ltd. |
Affiliation:
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Department of Neurosurgery, Kochi Medical School, Kochi University, Kohasu, Okoh-cho, Nankoku, Kochi 783-8505 Japan Laboratory of Human Health and Medical Science, Graduate School of Kuroshio Science, and Department of Molecular and Cellular Biology, Kochi Medical School, Kochi University, Kohasu, Okoh-cho, Nankoku, Kochi 783-8505 Japan Laboratory of Immunology, Faculty of Pharmacy, Osaka Ohtani University, 3-11-1, Nishikiorikita, Tondabayashi, Osaka 584-8540, Japan Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2217-14, Hayashi-machi, Takamatsu, Kagawa 761-0395, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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