Document Detail

Regulatory effects of Spirulina complex polysaccharides on growth of murine RSV-M glioma cells through toll-like receptor 4.
MedLine Citation:
PMID:  23134155     Owner:  NLM     Status:  Publisher    
The present study is the first to describe the suppressive effect of Spirulina complex polysaccharides (CPS) on glioma growth by down-regulating angiogenesis via a Toll-like receptor (TLR) 4 signal. Murine RSV-M glioma cells were implanted subcutaneously (S.C.) in C3H/HeN mice and into TLR4 mutant C3H/HeJ mice. Treatment with either Spirulina CPS or Escherichia coli (E. coli) lipopolysaccharides (LPS) strongly suppressed RSV-M glioma cell growth in C3H/HeN mice but not C3H/HeJ. Glioma cells stimulated production of interleukin (IL)-17 in both tumor-bearing C3H/HeN and C3H/HeJ mice. Treatment of E. coli LPS induced much higher level of IL-17 production in tumor-bearing C3H/HeN mice compared to tumor-bearing C3H/HeJ mice. In C3H/HeN mice, treatment with Spirulina CPS but not with E. coli LPS suppressed growth of re-transplanted glioma, suggesting that Spirulina CPS enhances the immune response. Administration of anti-CD8 antibodies, anti-CD4 and anti-CD8 antibodies or anti-asialo GM1 antibodies enhanced the tumor growth, suggesting that T cells, and natural killer (NK) cells or macrophages were involved in the suppression of the tumor growth by Spirulina CPS. Although anti-interferon (IFN)-γ antibodies had no effect on glioma cell growth, anti-IL-17 antibodies administered four days after tumor transplantation suppressed growth similarly to treatment with Spirulina CPS. Lower level of angiogenesis was observed in gliomas from Spirulina CPS-treated mice compared to those from saline- or E. coli LPS-treated mice. These results suggest that Spirulina CPS antagonizes glioma cell growth by down-regulating angiogenesis, in part, by regulating IL-17 production in C3H/HeN mice.
Yu Kawanishi; Akira Tominaga; Hiromi Okuyama; Satoshi Fukuoka; Takahiro Taguchi; Yutaka Kusumoto; Toshio Yawata; Yasunori Fujimoto; Shiro Ono; Keiji Shimizu
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-8
Journal Detail:
Title:  Microbiology and immunology     Volume:  -     ISSN:  1348-0421     ISO Abbreviation:  Microbiol. Immunol.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-8     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7703966     Medline TA:  Microbiol Immunol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 The Societies and Wiley Publishing Asia Pty Ltd.
Department of Neurosurgery, Kochi Medical School, Kochi University, Kohasu, Okoh-cho, Nankoku, Kochi 783-8505 Japan Laboratory of Human Health and Medical Science, Graduate School of Kuroshio Science, and Department of Molecular and Cellular Biology, Kochi Medical School, Kochi University, Kohasu, Okoh-cho, Nankoku, Kochi 783-8505 Japan Laboratory of Immunology, Faculty of Pharmacy, Osaka Ohtani University, 3-11-1, Nishikiorikita, Tondabayashi, Osaka 584-8540, Japan Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2217-14, Hayashi-machi, Takamatsu, Kagawa 761-0395, Japan.
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