Document Detail

Regulatory characteristics of amino acid transport in newborn rat renal cortex cells.
MedLine Citation:
PMID:  1268209     Owner:  NLM     Status:  MEDLINE    
The uptaken of alpha-aminoisobutyric acid by slices of kidney cortex from newborn rats is enhanced by a preliminary incubation of the tissue in buffer at 37 degrees C. This effect is abolished by anaerobiosis, the presence of dinitrophenol or the removal of Na+ during the preliminary incubation. Cycloheximide (50 muM) and purimycin (1 mM) as well as alpha-aminoisobutyric acid, glycine and proline (5 mM) in the preincubation buffer also abolish the effect, while actinomycin D (0.8 muM) partially the phenomenon indicates that the enhanced uptake is due to an increased entry rate into the cells without a change in effux. There is no alteration in the apparent transport Km but an increase in the V for entry. The effect is dependent on tissue age being observed between birth and 22 days, after which there is a decrease in response to preliminary incubation with no effect seen in adult tissues.
R A Reynolds; S Segal
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  426     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1976 Mar 
Date Detail:
Created Date:  1976-08-02     Completed Date:  1976-08-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  513-25     Citation Subset:  IM    
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MeSH Terms
Amino Acids / metabolism*
Aminoisobutyric Acids / metabolism
Animals, Newborn
Biological Transport, Active
Cycloheximide / pharmacology
Cycloleucine / metabolism
Dactinomycin / pharmacology
Dinitrophenols / pharmacology
Glycine / metabolism
Kidney Cortex / drug effects,  growth & development,  metabolism*
Ouabain / pharmacology
Proline / metabolism
Puromycin / pharmacology
Sodium / pharmacology
Reg. No./Substance:
0/Amino Acids; 0/Aminoisobutyric Acids; 0/Dinitrophenols; 147-85-3/Proline; 50-76-0/Dactinomycin; 52-52-8/Cycloleucine; 53-79-2/Puromycin; 56-40-6/Glycine; 630-60-4/Ouabain; 66-81-9/Cycloheximide; 7440-23-5/Sodium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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