| Regulatory characteristics of amino acid transport in newborn rat renal cortex cells. | |
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MedLine Citation:
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PMID: 1268209 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The uptaken of alpha-aminoisobutyric acid by slices of kidney cortex from newborn rats is enhanced by a preliminary incubation of the tissue in buffer at 37 degrees C. This effect is abolished by anaerobiosis, the presence of dinitrophenol or the removal of Na+ during the preliminary incubation. Cycloheximide (50 muM) and purimycin (1 mM) as well as alpha-aminoisobutyric acid, glycine and proline (5 mM) in the preincubation buffer also abolish the effect, while actinomycin D (0.8 muM) partially the phenomenon indicates that the enhanced uptake is due to an increased entry rate into the cells without a change in effux. There is no alteration in the apparent transport Km but an increase in the V for entry. The effect is dependent on tissue age being observed between birth and 22 days, after which there is a decrease in response to preliminary incubation with no effect seen in adult tissues. |
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Authors:
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R A Reynolds; S Segal |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 426 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1976 Mar |
Date Detail:
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Created Date: 1976-08-02 Completed Date: 1976-08-02 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 513-25 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aging Amino Acids / metabolism* Aminoisobutyric Acids / metabolism Anaerobiosis Animals Animals, Newborn Biological Transport, Active Cycloheximide / pharmacology Cycloleucine / metabolism Dactinomycin / pharmacology Dinitrophenols / pharmacology Glycine / metabolism Kidney Cortex / drug effects, growth & development, metabolism* Kinetics Ouabain / pharmacology Proline / metabolism Puromycin / pharmacology Rats Sodium / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Aminoisobutyric Acids; 0/Dinitrophenols; 147-85-3/Proline; 50-76-0/Dactinomycin; 52-52-8/Cycloleucine; 53-79-2/Puromycin; 56-40-6/Glycine; 630-60-4/Ouabain; 66-81-9/Cycloheximide; 7440-23-5/Sodium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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