| Regulatory changes of apoptotic factors in the bovine corpus luteum after induced luteolysis. | |
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MedLine Citation:
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PMID: 18563705 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The corpus luteum (CL) offers the opportunity to study not only proliferative, but also regressive processes. During luteolysis of the CL a sudden death of luteal and endothelial cells seems to be involved (apoptosis). The aim of this study was to examen the mRNA expression of factors known to be involved in apoptotic processes: monocyte chemoattractant protein-1 (MCP-1), factors of the extrinsic and intrinsic apoptotic pathways, caspase3, -6, -7 and interferone gamma (IFNgamma). Luteolysis was induced by injection of 500 microg Cloprostenol during mid-luteal phase. The CLs were collected at 0.5, 2, 4, 12, 24, 48, and 64 hr after PGF2alpha-injection. Control CLs (Days 8-12) were collected at the slaugtherhouse. Real-time RT-PCR determined the mRNA expressions. Western blot analysis of poly(ADP-ribose) polymerase (PARP-1) and IFNgamma as well as protein measurement of tumor necrosis factor alpha (TNFalpha) by EIA were performed. The mRNA levels of MCP-1, IFNgamma and most factors of the extrinsic pathway were significantly increased between 0.5 and 2 hr. The factors of the intrinsic pathway were mostly later up-regulated at 24-48 hr after PGF2alpha. Caspase6 and 3 revealed a significant increase from 2 and 12 hr, respectively, whereas caspase7 was significantly up-regulated after 24 hr. The protein level of TNFalpha increased significantly to a maximum level at 12 hr. The Western blot revealed an increasing level of an 89 kDa fragment of PARP-1 from 12 to 24 hr, which is specific for apoptosis. We assume that the extrinsic pathway is more important for the onset of luteolysis, because of its earlier and higher increase during induced luteolysis. |
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Authors:
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H Kliem; B Berisha; H H D Meyer; D Schams |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Molecular reproduction and development Volume: 76 ISSN: 1098-2795 ISO Abbreviation: Mol. Reprod. Dev. Publication Date: 2009 Mar |
Date Detail:
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Created Date: 2009-01-26 Completed Date: 2009-08-07 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8903333 Medline TA: Mol Reprod Dev Country: United States |
Other Details:
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Languages: eng Pagination: 220-30 Citation Subset: IM |
Affiliation:
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Physiology Weihenstephan, Technical University Munich, Freising, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens, CD / genetics, metabolism Antigens, Differentiation, Myelomonocytic / genetics, metabolism Apoptosis / physiology Apoptosis Regulatory Proteins / genetics*, metabolism* Caspases / genetics, metabolism Cattle Chemokine CCL2 / genetics, metabolism Cloprostenol / pharmacology Corpus Luteum / chemistry Female Gene Expression Regulation / drug effects, physiology* Interferon-gamma / genetics, metabolism Luteolysis / drug effects, physiology* Luteolytic Agents / pharmacology Poly(ADP-ribose) Polymerases / metabolism Progesterone / blood Proto-Oncogene Proteins c-bcl-2 / genetics, metabolism Receptors, CCR2 / genetics, metabolism Receptors, Cell Surface / genetics, metabolism Tumor Necrosis Factor-alpha / genetics, metabolism Tumor Suppressor Protein p53 / genetics, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD; 0/Antigens, Differentiation, Myelomonocytic; 0/Apoptosis Regulatory Proteins; 0/CD163 antigen; 0/Chemokine CCL2; 0/Luteolytic Agents; 0/Proto-Oncogene Proteins c-bcl-2; 0/Receptors, CCR2; 0/Receptors, Cell Surface; 0/Tumor Necrosis Factor-alpha; 0/Tumor Suppressor Protein p53; 40665-92-7/Cloprostenol; 57-83-0/Progesterone; 82115-62-6/Interferon-gamma; EC 2.4.2.30/Poly(ADP-ribose) Polymerases; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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