Document Detail

Regulatory control of the resolution of DNA recombination intermediates during meiosis and mitosis.
MedLine Citation:
PMID:  21962513     Owner:  NLM     Status:  MEDLINE    
The efficient and timely resolution of DNA recombination intermediates is essential for bipolar chromosome segregation. Here, we show that the specialized chromosome segregation patterns of meiosis and mitosis, which require the coordination of recombination with cell-cycle progression, are achieved by regulating the timing of activation of two crossover-promoting endonucleases. In yeast meiosis, Mus81-Mms4 and Yen1 are controlled by phosphorylation events that lead to their sequential activation. Mus81-Mms4 is hyperactivated by Cdc5-mediated phosphorylation in meiosis I, generating the crossovers necessary for chromosome segregation. Yen1 is also tightly regulated and is activated in meiosis II to resolve persistent Holliday junctions. In yeast and human mitotic cells, a similar regulatory network restrains these nuclease activities until mitosis, biasing the outcome of recombination toward noncrossover products while also ensuring the elimination of any persistent joint molecules. Mitotic regulation thereby facilitates chromosome segregation while limiting the potential for loss of heterozygosity and sister-chromatid exchanges.
Joao Matos; Miguel G Blanco; Sarah Maslen; J Mark Skehel; Stephen C West
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell     Volume:  147     ISSN:  1097-4172     ISO Abbreviation:  Cell     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-10-03     Completed Date:  2011-11-30     Revised Date:  2014-10-22    
Medline Journal Info:
Nlm Unique ID:  0413066     Medline TA:  Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  158-72     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier Inc. All rights reserved.
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MeSH Terms
Cell Cycle
Crossing Over, Genetic
DNA, Cruciform*
HeLa Cells
Holliday Junction Resolvases / metabolism
Recombination, Genetic*
Saccharomyces cerevisiae / cytology*,  enzymology,  metabolism*
Grant Support
11582//Cancer Research UK; 249145//European Research Council; //Cancer Research UK
Reg. No./Substance:
0/DNA, Cruciform; EC 3.1.21.-/Holliday Junction Resolvases

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