Document Detail


Regulators of G protein signaling RGS7 and RGS11 determine the onset of the light response in ON bipolar neurons.
MedLine Citation:
PMID:  22547806     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The time course of signaling via heterotrimeric G proteins is controlled through their activation by G-protein coupled receptors and deactivation through the action of GTPase accelerating proteins (GAPs). Here we identify RGS7 and RGS11 as the key GAPs in the mGluR6 pathway of retinal rod ON bipolar cells that set the sensitivity and time course of light-evoked responses. We showed using electroretinography and single cell recordings that the elimination of RGS7 did not influence dark-adapted light-evoked responses, but the concurrent elimination of RGS11 severely reduced their magnitude and dramatically slowed the onset of the response. In RGS7/RGS11 double-knockout mice, light-evoked responses in rod ON bipolar cells were only observed during persistent activation of rod photoreceptors that saturate rods. These observations are consistent with persistently high G-protein activity in rod ON bipolar cell dendrites caused by the absence of the dominant GAP, biasing TRPM1 channels to the closed state.
Authors:
Yan Cao; Johan Pahlberg; Ignacio Sarria; Naomi Kamasawa; Alapakkam P Sampath; Kirill A Martemyanov
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-04-30
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  109     ISSN:  1091-6490     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-05-16     Completed Date:  2012-07-27     Revised Date:  2013-06-25    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  7905-10     Citation Subset:  IM    
Affiliation:
Department of Neuroscience, The Scripps Research Institute, Jupiter, FL 33458, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blotting, Western
Electroretinography
GTPase-Activating Proteins / genetics,  metabolism*
Immunohistochemistry
Light Signal Transduction / physiology*
Mice
Mice, Knockout
Microscopy, Electron, Transmission
Patch-Clamp Techniques
Photic Stimulation
RGS Proteins / genetics,  metabolism*
Retinal Bipolar Cells / metabolism,  physiology*
Retinal Rod Photoreceptor Cells / metabolism,  physiology*
Signal Transduction / physiology*
Grant Support
ID/Acronym/Agency:
EY017606/EY/NEI NIH HHS; NS059934/NS/NINDS NIH HHS; R01 EY017606/EY/NEI NIH HHS; R01 EY018139/EY/NEI NIH HHS
Chemical
Reg. No./Substance:
0/GTPase-Activating Proteins; 0/RGS Proteins; 0/Rgs11 protein, mouse; 0/Rgs7 protein, mouse
Comments/Corrections

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