Document Detail

Regulation of a viral proteinase by a peptide and DNA in one-dimensional space: IV. viral proteinase slides along DNA to locate and process its substrates.
MedLine Citation:
PMID:  23043138     Owner:  NLM     Status:  MEDLINE    
Precursor proteins used in the assembly of adenovirus virions must be processed by the virally encoded adenovirus proteinase (AVP) before the virus particle becomes infectious. An activated adenovirus proteinase, the AVP-pVIc complex, was shown to slide along viral DNA with an extremely fast one-dimensional diffusion constant, 21.0 ± 1.9 × 10(6) bp(2)/s. In principle, one-dimensional diffusion can provide a means for DNA-bound proteinases to locate and process DNA-bound substrates. Here, we show that this is correct. In vitro, AVP-pVIc complexes processed a purified virion precursor protein in a DNA-dependent reaction; in a quasi in vivo environment, heat-disrupted ts-1 virions, AVP-pVIc complexes processed five different precursor proteins in DNA-dependent reactions. Sliding of AVP-pVIc complexes along DNA illustrates a new biochemical mechanism by which a proteinase can locate its substrates, represents a new paradigm for virion maturation, and reveals a new way of exploiting the surface of DNA.
Paul C Blainey; Vito Graziano; Ana J Pérez-Berná; William J McGrath; S Jane Flint; Carmen San Martín; X Sunney Xie; Walter F Mangel
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-10-07
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  288     ISSN:  1083-351X     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-01-21     Completed Date:  2013-03-26     Revised Date:  2014-01-24    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2092-102     Citation Subset:  IM    
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MeSH Terms
Adenoviruses, Human / enzymology*,  genetics
Amino Acid Sequence
Capsid Proteins / chemistry*,  genetics,  metabolism
Cysteine Endopeptidases / chemistry*,  genetics,  metabolism
DNA, Viral / chemistry*,  metabolism
Enzyme Activation
Escherichia coli / genetics
Hot Temperature
Models, Molecular
Molecular Sequence Data
Protein Binding
Protein Precursors / chemistry*,  genetics,  metabolism
Protein Structure, Secondary
Protein Structure, Tertiary
Recombinant Proteins / chemistry,  genetics,  metabolism
Virion / enzymology*,  genetics
Grant Support
Reg. No./Substance:
0/Capsid Proteins; 0/DNA, Viral; 0/Protein Precursors; 0/Recombinant Proteins; 0/polypeptide VI, adenovirus; EC 3.4.22.-/Cysteine Endopeptidases; EC

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