Document Detail


Regulation of a viral proteinase by a peptide and DNA in one-dimensional space: I. binding to DNA AND to hexon of the precursor to protein VI, pVI, of human adenovirus.
MedLine Citation:
PMID:  23043136     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The precursor to adenovirus protein VI, pVI, is a multifunctional protein with different roles early and late in virus infection. Here, we focus on two roles late in infection, binding of pVI to DNA and to the major capsid protein hexon. pVI bound to DNA as a monomer independent of DNA sequence with an apparent equilibrium dissociation constant, K(d)((app)), of 46 nm. Bound to double-stranded DNA, one molecule of pVI occluded 8 bp. Upon the binding of pVI to DNA, three sodium ions were displaced from the DNA. A ΔG(0)(0) of -4.54 kcal/mol for the nonelectrostatic free energy of binding indicated that a substantial component of the binding free energy resulted from nonspecific interactions between pVI and DNA. The proteolytically processed, mature form of pVI, protein VI, also bound to DNA; its K(d)((app)) was much higher, 307 nm. The binding assays were performed in 1 mm MgCl(2) because in the absence of magnesium, the binding to pVI or protein VI to DNA was too tight to determine a K(d)((app)). Three molecules of pVI bound to one molecule of the hexon trimer with an equilibrium dissociation constant K(d)((app)) of 1.1 nm.
Authors:
Vito Graziano; William J McGrath; Maarit Suomalainen; Urs F Greber; Paul Freimuth; Paul C Blainey; Guobin Luo; X Sunney Xie; Walter F Mangel
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-10-07
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  288     ISSN:  1083-351X     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-01-21     Completed Date:  2013-03-26     Revised Date:  2014-01-24    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2059-67     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adenoviruses, Human / genetics,  metabolism*
Amino Acid Sequence
Capsid / chemistry
Capsid Proteins / chemistry,  genetics,  metabolism*
Cations, Monovalent
Cysteine Endopeptidases / chemistry,  genetics,  metabolism*
DNA, Viral / chemistry,  metabolism
Escherichia coli / genetics
HeLa Cells
Humans
Kinetics
Magnesium Chloride / chemistry
Molecular Sequence Data
Protein Precursors / chemistry,  genetics,  metabolism*
Recombinant Proteins / chemistry,  genetics,  metabolism
Sodium / chemistry
Thermodynamics
Grant Support
ID/Acronym/Agency:
DP1 OD000277/OD/NIH HHS; R01AI41599/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Capsid Proteins; 0/Cations, Monovalent; 0/DNA, Viral; 0/Protein Precursors; 0/Recombinant Proteins; 0/hexon capsid protein, Adenovirus; 0/polypeptide VI, adenovirus; 02F3473H9O/Magnesium Chloride; 9NEZ333N27/Sodium; EC 3.4.22.-/Cysteine Endopeptidases; EC 3.4.22.39/adenain
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