Document Detail

Regulation of various proteolytic pathways by insulin and amino acids in human fibroblasts.
MedLine Citation:
PMID:  17610878     Owner:  NLM     Status:  MEDLINE    
Intracellular protein degradation is a regulated process with several proteolytic pathways. Although regulation of macroautophagy has been investigated in some detail in hepatocytes and in few other cells, less is known on this regulation in other cells and proteolytic pathways. We show that in human fibroblasts insulin and amino acids reduce protein degradation by different signalling pathways and that this inhibition proceeds in part via the mammalian target of rapamycin, especially with amino acids, which probably increase lysosomal pH. Moreover, the regulatory amino acids (Phe, Arg, Met, Tyr, Trp and Cys) are partially different from other cells. Finally, and in addition to macroautophagy, insulin and amino acids modify, to different extents and sometimes in opposite directions, the activities of other proteolytic pathways.
Inmaculada Esteban; Carmen Aguado; Maribel Sánchez; Erwin Knecht
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-06-27
Journal Detail:
Title:  FEBS letters     Volume:  581     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-08-13     Completed Date:  2007-09-19     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  3415-21     Citation Subset:  IM    
Laboratorio de Biología Celular, Centro de Investigación Príncipe Felipe, Avda. Autopista del Saler 16, 46013-Valencia, Spain.
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MeSH Terms
Amino Acids / pharmacology*
Cells, Cultured
Insulin / pharmacology*
Microscopy, Electron
Peptide Hydrolases / metabolism*
Protein Kinases / metabolism
Reg. No./Substance:
0/Amino Acids; 11061-68-0/Insulin; EC 2.7.-/Protein Kinases; EC 2.7.1.-/mTOR protein; EC 3.4.-/Peptide Hydrolases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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