Document Detail


Regulation of tryptophan operon expression in the archaeon Methanothermobacter thermautotrophicus.
MedLine Citation:
PMID:  16159776     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Conserved trp genes encode enzymes that catalyze tryptophan biosynthesis in all three biological domains, and studies of their expression in Bacteria and eukaryotes have revealed a variety of different regulatory mechanisms. The results reported here provide the first detailed description of an archaeal trp gene regulatory system. We have established that the trpEGCFBAD operon in Methanothermobacter thermautotrophicus is transcribed divergently from a gene (designated trpY) that encodes a tryptophan-sensitive transcription regulator. TrpY binds to TRP box sequences (consensus, TGTACA) located in the overlapping promoter regions between trpY and trpE, inhibiting trpY transcription in the absence of tryptophan and both trpY and trpEGCFBAD transcription in the presence of tryptophan. TrpY apparently inhibits trpY transcription by blocking RNA polymerase access to the site of trpY transcription initiation and represses trpEGCFBAD transcription by preventing TATA box binding protein (TBP) binding to the TATA box sequence. Given that residue 2 (W2) is the only tryptophan in TrpY and in TrpY homologues in other Euryarchaea and that there is only one tryptophan codon in the entire trpEGCFBAD operon (trpB encodes W175), expression of the trp operon may also be regulated in vivo by the supply of charged tRNA(Trp) available to translate the second codon of the trpY mRNA.
Authors:
Yunwei Xie; John N Reeve
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of bacteriology     Volume:  187     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  2005 Sep 
Date Detail:
Created Date:  2005-09-14     Completed Date:  2005-11-14     Revised Date:  2013-06-07    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6419-29     Citation Subset:  IM    
Affiliation:
Department of Microbiology, Ohio State University, Columbus, Ohio 43210-1292, USA.
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MeSH Terms
Descriptor/Qualifier:
Euryarchaeota / genetics*
Gene Expression Regulation, Archaeal*
Multigene Family / genetics*
Operon / genetics*
Tryptophan / biosynthesis,  genetics*
Grant Support
ID/Acronym/Agency:
GM53185/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
73-22-3/Tryptophan
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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