Document Detail


Regulation of tropomyosin gene expression during myogenesis.
MedLine Citation:
PMID:  6086009     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In skeletal muscle, tropomyosin has a critical role in transduction of calcium-induced contraction. Presently, little is known about the regulation of tropomyosin gene expression during myogenesis. In the present study, qualitative and quantitative changes in the nucleic acid populations of differentiating chicken embryo muscle cells in culture have been examined. Total nucleic acid content per nucleus increased about fivefold in fully developed myotubes as compared to mononucleated myoblasts. The contribution of deoxyribonucleic acid to the total nucleic acid population decreased from 24% in myoblasts to 5% of total nucleic acid in myotubes. Concomitant with the decrement in deoxyribonucleic acid contribution to total nucleic acid was an increase in polyadenylated ribonucleic acid (RNA) content per cell which reached levels in myotubes that were 17-fold higher than those of myoblasts. Specific changes in the RNA population during myogenesis were further investigated by quantitation of the synthetic capacity (messenger RNA levels) per cell for alpha- and beta-tropomyosin. Cell-free translation and immunoprecipitation demonstrated an approximately 40-fold increase in messenger RNA levels per nucleus for alpha- and beta-tropomyosin after fusion in the terminally differentiated myotubes. Indirect immunofluorescence with affinity-purified tropomyosin antibodies demonstrated the presence of tropomyosin-containing filaments in cells throughout myogenesis. Thus, the tropomyosin genes are constitutively expressed during muscle differentiation through the production of tropomyosin messenger RNA and translation into tropomyosin protein.
Authors:
M Moss; R Schwartz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular and cellular biology     Volume:  1     ISSN:  0270-7306     ISO Abbreviation:  Mol. Cell. Biol.     Publication Date:  1981 Mar 
Date Detail:
Created Date:  1988-02-12     Completed Date:  1988-02-12     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  8109087     Medline TA:  Mol Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  289-301     Citation Subset:  IM    
Affiliation:
Department of Cell Biology, Baylor College of Medicine, Texas Medical Center, Houston 77030.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cells, Cultured
Gene Expression Regulation*
Muscle Development
Muscles / metabolism*
Poly A / metabolism
Protein Biosynthesis
RNA / metabolism
RNA, Messenger / genetics,  metabolism
Tropomyosin / biosynthesis,  genetics*
Grant Support
ID/Acronym/Agency:
NS 15050/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/RNA, Messenger; 0/Tropomyosin; 24937-83-5/Poly A; 63231-63-0/RNA
Comments/Corrections

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