| Regulation of trophoblast invasion by IL-1beta and TGF-beta1. | |
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MedLine Citation:
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PMID: 12516631 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PROBLEM: To investigate the effect of IL-1beta and TGF-beta1 on trophoblast derived proteases and their inhibitors at the mRNA and protein level for elucidation of the mechanism of trophoblast invasion. METHOD OF STUDY: Trophoblast derived proteases and their inhibitors were localized in human placental villi by immunohistochemistry, their regulation at mRNA and protein levels were studied using RT-PCR and zymography, respectively, on trophoblast cells in culture. RESULTS: Trophoblast proteases, matrix metalloproteases (MMP-2, MMP-9), membrane type matrix metalloproteases (MT-MMP1 & 2) and urokinase type plasminogen activator (uPA) were found to be up regulated by IL-1beta while the protease inhibitors, tissue inhibitor of metalloproteases (TIMP-1 & 2) and plasminogen activator inhibitors (PAI-1 & 2) to be up regulated by TGF-beta1. CONCLUSION: Temporo-spatial regulation of trophoblast invasion is the outcome of a balanced interplay between the proteases and their cognate inhibitors. IL-1beta and TGF-beta1 seem to be critical for regulating the protease network thereby effectively controlling the extent to which the trophoblast may invade the maternal endometrium. |
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Authors:
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Subhradip Karmakar; Chandana Das |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: American journal of reproductive immunology (New York, N.Y. : 1989) Volume: 48 ISSN: 1046-7408 ISO Abbreviation: Am. J. Reprod. Immunol. Publication Date: 2002 Oct |
Date Detail:
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Created Date: 2003-01-08 Completed Date: 2003-04-16 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8912860 Medline TA: Am J Reprod Immunol Country: Denmark |
Other Details:
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Languages: eng Pagination: 210-9 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, All India Institute of Medical Sciences, Ansari Nagar, New Delhi 110029, India. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Culture Media, Conditioned
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metabolism Embryo Implantation / physiology* Endopeptidases / metabolism* Immunohistochemistry Interleukin-1 / metabolism* Matrix Metalloproteinase 2 / genetics Matrix Metalloproteinase 9 / genetics Reverse Transcriptase Polymerase Chain Reaction Tissue Inhibitor of Metalloproteinase-1 / genetics Tissue Inhibitor of Metalloproteinase-2 / genetics Transforming Growth Factor beta / metabolism* Transforming Growth Factor beta1 Trophoblasts / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Culture Media, Conditioned; 0/Interleukin-1; 0/TGFB1 protein, human; 0/Tissue Inhibitor of Metalloproteinase-1; 0/Transforming Growth Factor beta; 0/Transforming Growth Factor beta1; 127497-59-0/Tissue Inhibitor of Metalloproteinase-2; EC 3.4.-/Endopeptidases; EC 3.4.24.24/Matrix Metalloproteinase 2; EC 3.4.24.35/Matrix Metalloproteinase 9 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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