Document Detail


Regulation of transcription by unnatural amino acids.
MedLine Citation:
PMID:  21240267     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Small-molecule regulation of gene expression is intrinsic to cellular function and indispensable to the construction of new biological sensing, control and expression systems. However, there are currently only a handful of strategies for engineering such regulatory components and fewer still that can give rise to an arbitrarily large set of inducible systems whose members respond to different small molecules, display uniformity and modularity in their mechanisms of regulation, and combine to actuate universal logics. Here we present an approach for small-molecule regulation of transcription based on the combination of cis-regulatory leader-peptide elements with genetically encoded unnatural amino acids (amino acids that have been artificially added to the genetic code). In our system, any genetically encoded unnatural amino acid (UAA) can be used as a small-molecule attenuator or activator of gene transcription, and the logics intrinsic to the network defined by expanded genetic codes can be actuated.
Authors:
Chang C Liu; Lei Qi; Charles Yanofsky; Adam P Arkin
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-1-16
Journal Detail:
Title:  Nature biotechnology     Volume:  -     ISSN:  1546-1696     ISO Abbreviation:  -     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2011-1-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9604648     Medline TA:  Nat Biotechnol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
[1] Department of Bioengineering, University of California at Berkeley, Berkeley, California, USA. [2] Miller Institute for Basic Research in Science, Berkeley, California, USA.
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