Document Detail

Regulation of transcription by unnatural amino acids.
MedLine Citation:
PMID:  21240267     Owner:  NLM     Status:  Publisher    
Small-molecule regulation of gene expression is intrinsic to cellular function and indispensable to the construction of new biological sensing, control and expression systems. However, there are currently only a handful of strategies for engineering such regulatory components and fewer still that can give rise to an arbitrarily large set of inducible systems whose members respond to different small molecules, display uniformity and modularity in their mechanisms of regulation, and combine to actuate universal logics. Here we present an approach for small-molecule regulation of transcription based on the combination of cis-regulatory leader-peptide elements with genetically encoded unnatural amino acids (amino acids that have been artificially added to the genetic code). In our system, any genetically encoded unnatural amino acid (UAA) can be used as a small-molecule attenuator or activator of gene transcription, and the logics intrinsic to the network defined by expanded genetic codes can be actuated.
Chang C Liu; Lei Qi; Charles Yanofsky; Adam P Arkin
Related Documents :
8930137 - Fatty acid modification of membrane fluidity in chinese hamster ovary (tr715-19) cells.
2586237 - Plasma lipoproteins in dairy cows with naturally occurring severe fatty liver: evidence...
17972107 - Climatic control of trophic interaction strength: the effect of lizards on spiders.
176717 - Studies on human serum high-density lipoproteins. v. isolation and characterization of ...
21554327 - Revealing complexity and specificity in the activation of lipase-mediated oxylipin bios...
1547187 - Pathogenesis of carbohydrate-induced hypertriglyceridemia using hepg2 cells as a model ...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-1-16
Journal Detail:
Title:  Nature biotechnology     Volume:  -     ISSN:  1546-1696     ISO Abbreviation:  -     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2011-1-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9604648     Medline TA:  Nat Biotechnol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
[1] Department of Bioengineering, University of California at Berkeley, Berkeley, California, USA. [2] Miller Institute for Basic Research in Science, Berkeley, California, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Efficient mucosal vaccination mediated by the neonatal Fc receptor.
Next Document:  Structure of UvrA nucleotide excision repair protein in complex with modified DNA.