Document Detail


Regulation of telomerase and its hTERT messenger in colorectal cancer.
MedLine Citation:
PMID:  14719074     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Telomeres are the distal ends of human chromosomes composed of tandem repeats of the sequence TTAGGG. In most human somatic cells, telomerase activity is undetectable, and the telomere length is progressively shortened during cell proliferation, leading to cellular senescence. In contrast, telomerase is activated in the vast majority of cancer cells, including colorectal cancer. The human telomerase complex is comprised of multiple components, but telomerase reverse transcriptase (hTERT) is the most important component for the control of telomerase activity. The p53 protein is a transcription factor with multiple biological activities, including cell cycle arrest and/or apoptosis upon DNA damage, hypoxia and oncogene activation; this requires transactivation or repression of specific target genes by wild-type p53. To better understand if a link between hTERT/telomerase regulation and p53 status exists in colorectal carcinogenesis, we analysed 43 cases of colorectal carcinoma for hTERT mRNA expression and telomerase activity. Moreover, a complete analysis of p53 status was performed. Alterations of p53 gene were found in 44.19% of cases and missense point mutations represented a high proportion of p53. Both telomerase activity (p=0.014) and hTERT expression (p=0.03) were significantly associated with p53 mutations, suggesting a role of p53 in the signaling pathway for telomerase control.
Authors:
Laura Boldrini; Pinuccia Faviana; Silvia Gisfredi; Valentina Donati; Ylenia Zucconi; Silvia Ursino; Paolo Simi; Fulvia Baldinotti; Piero Berti; Davide Galleri; Gabriele Materazzi; Fulvio Basolo; Paolo Miccoli; Raffaele Pingitore; Gabriella Fontanini
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Oncology reports     Volume:  11     ISSN:  1021-335X     ISO Abbreviation:  Oncol. Rep.     Publication Date:  2004 Feb 
Date Detail:
Created Date:  2004-01-13     Completed Date:  2004-08-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9422756     Medline TA:  Oncol Rep     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  395-400     Citation Subset:  IM    
Affiliation:
Department of Oncology, Transplants and Advanced Technologies in Medicine, University of Pisa, Via Roma 57, I-56126 Pisa, Italy.
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MeSH Terms
Descriptor/Qualifier:
Adenocarcinoma / genetics,  pathology,  surgery
Amino Acid Substitution
Codon / genetics
Colonic Neoplasms / genetics*,  surgery
Colorectal Neoplasms / genetics*,  surgery
DNA, Neoplasm / genetics,  isolation & purification
DNA-Binding Proteins
Gene Expression Regulation, Enzymologic / genetics
Gene Expression Regulation, Neoplastic / genetics*
Genes, p53 / genetics
Humans
Polymerase Chain Reaction
Polymorphism, Single-Stranded Conformational
RNA, Messenger / genetics*
Repetitive Sequences, Nucleic Acid
Sequence Deletion
Telomerase / genetics*
Transcription, Genetic / genetics
Tumor Suppressor Protein p53 / genetics
Chemical
Reg. No./Substance:
0/Codon; 0/DNA, Neoplasm; 0/DNA-Binding Proteins; 0/RNA, Messenger; 0/Tumor Suppressor Protein p53; EC 2.7.7.49/Telomerase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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