| Regulation of steroid production in ovarian stromal tissue from 5- to 8-week-old pullets and laying hens. | |
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MedLine Citation:
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PMID: 8182589 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Studies were conducted to evaluate the regulation of steroid production in dispersed cells from ovarian stromal tissue from 5- to 8-week-old-pullets (IM cells) and laying hens (MAT cells). Short-term incubation of IM and MAT cells with ovine (o) LH resulted in a dose-dependent increase in progesterone, androstenedione and oestradiol production; progesterone production was greater in MAT cells than in IM cells (P < 0.05) in response to 2-200 ng oLH ml-1, whereas androstenedione and oestradiol production was greater in MAT cells following treatment with 20 and 200 ng oLH ml-1 (P < 0.05). In both cell populations the cyclic adenosine monophosphate (cAMP) analogue, 8-bromo-cAMP (1 and 10 mmol l-1) stimulated progesterone and androstenedione production, whereas oLH (200 ng ml-1) and forskolin (1-10 mumol l-1) promoted cAMP accumulation (P < 0.05 compared with basal values). However, treatment with the protein kinase C activator, phorbol 12-myristate 13-acetate (PMA), did not alter basal or oLH-stimulated cAMP accumulation or progesterone production in either IM or MAT cells (P > 0.10). PMA did, however, inhibit agonist-induced androstenedione production (P < 0.05); co-treatment with the calcium ionophore A23187 potentiated this inhibitory effect. Finally, treatment with transforming growth factor-alpha (TGF-alpha; 1.8-18 pmol l-1) did not affect basal or oLH-stimulated progesterone or androstenedione production by IM cells, MAT cells, theca cells from 6-8 mm follicles or theca cells from the second largest (F2) follicle (P > 0.10).(ABSTRACT TRUNCATED AT 250 WORDS) |
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Authors:
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J M Levorse; A L Johnson |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Journal of reproduction and fertility Volume: 100 ISSN: 0022-4251 ISO Abbreviation: J. Reprod. Fertil. Publication Date: 1994 Jan |
Date Detail:
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Created Date: 1994-06-14 Completed Date: 1994-06-14 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0376367 Medline TA: J Reprod Fertil Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 195-202 Citation Subset: IM |
Affiliation:
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Department of Animal Sciences, Rutgers, State University of New Jersey, New Brunswick 08903. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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8-Bromo Cyclic Adenosine Monophosphate
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pharmacology Androstenedione / biosynthesis Animals Calcimycin / pharmacology Cells, Cultured Chickens / metabolism* Estradiol / biosynthesis Female Forskolin / pharmacology Gonadal Steroid Hormones / biosynthesis* Luteinizing Hormone / pharmacology Ovary / cytology, drug effects, metabolism* Progesterone / biosynthesis Sexual Maturation / physiology* Tetradecanoylphorbol Acetate / pharmacology Transforming Growth Factor alpha / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Gonadal Steroid Hormones; 0/Transforming Growth Factor alpha; 16561-29-8/Tetradecanoylphorbol Acetate; 23583-48-4/8-Bromo Cyclic Adenosine Monophosphate; 50-28-2/Estradiol; 52665-69-7/Calcimycin; 57-83-0/Progesterone; 63-05-8/Androstenedione; 66428-89-5/Forskolin; 9002-67-9/Luteinizing Hormone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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