Document Detail


Regulation of steroid production in ovarian stromal tissue from 5- to 8-week-old pullets and laying hens.
MedLine Citation:
PMID:  8182589     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Studies were conducted to evaluate the regulation of steroid production in dispersed cells from ovarian stromal tissue from 5- to 8-week-old-pullets (IM cells) and laying hens (MAT cells). Short-term incubation of IM and MAT cells with ovine (o) LH resulted in a dose-dependent increase in progesterone, androstenedione and oestradiol production; progesterone production was greater in MAT cells than in IM cells (P < 0.05) in response to 2-200 ng oLH ml-1, whereas androstenedione and oestradiol production was greater in MAT cells following treatment with 20 and 200 ng oLH ml-1 (P < 0.05). In both cell populations the cyclic adenosine monophosphate (cAMP) analogue, 8-bromo-cAMP (1 and 10 mmol l-1) stimulated progesterone and androstenedione production, whereas oLH (200 ng ml-1) and forskolin (1-10 mumol l-1) promoted cAMP accumulation (P < 0.05 compared with basal values). However, treatment with the protein kinase C activator, phorbol 12-myristate 13-acetate (PMA), did not alter basal or oLH-stimulated cAMP accumulation or progesterone production in either IM or MAT cells (P > 0.10). PMA did, however, inhibit agonist-induced androstenedione production (P < 0.05); co-treatment with the calcium ionophore A23187 potentiated this inhibitory effect. Finally, treatment with transforming growth factor-alpha (TGF-alpha; 1.8-18 pmol l-1) did not affect basal or oLH-stimulated progesterone or androstenedione production by IM cells, MAT cells, theca cells from 6-8 mm follicles or theca cells from the second largest (F2) follicle (P > 0.10).(ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
J M Levorse; A L Johnson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of reproduction and fertility     Volume:  100     ISSN:  0022-4251     ISO Abbreviation:  J. Reprod. Fertil.     Publication Date:  1994 Jan 
Date Detail:
Created Date:  1994-06-14     Completed Date:  1994-06-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376367     Medline TA:  J Reprod Fertil     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  195-202     Citation Subset:  IM    
Affiliation:
Department of Animal Sciences, Rutgers, State University of New Jersey, New Brunswick 08903.
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MeSH Terms
Descriptor/Qualifier:
8-Bromo Cyclic Adenosine Monophosphate / pharmacology
Androstenedione / biosynthesis
Animals
Calcimycin / pharmacology
Cells, Cultured
Chickens / metabolism*
Estradiol / biosynthesis
Female
Forskolin / pharmacology
Gonadal Steroid Hormones / biosynthesis*
Luteinizing Hormone / pharmacology
Ovary / cytology,  drug effects,  metabolism*
Progesterone / biosynthesis
Sexual Maturation / physiology*
Tetradecanoylphorbol Acetate / pharmacology
Transforming Growth Factor alpha / pharmacology
Chemical
Reg. No./Substance:
0/Gonadal Steroid Hormones; 0/Transforming Growth Factor alpha; 16561-29-8/Tetradecanoylphorbol Acetate; 23583-48-4/8-Bromo Cyclic Adenosine Monophosphate; 50-28-2/Estradiol; 52665-69-7/Calcimycin; 57-83-0/Progesterone; 63-05-8/Androstenedione; 66428-89-5/Forskolin; 9002-67-9/Luteinizing Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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