| Regulation of the stability of cell surface E-cadherin by the proteasome. | |
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MedLine Citation:
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PMID: 19245796 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The epithelial-mesenchymal transition (EMT), a crucial event in cancer progression and embryonic development, is induced by transforming growth factor (TGF)-beta. Expression of E-cadherin, a representative epithelial marker, is repressed through transcriptional reduction by TGF-beta. Here, we show that endocytosis of cell surface E-cadherin during EMT induced by TGF-beta and during scattering induced by hepatocyte growth factor (HGF) can be blocked by inhibiting proteasome with lactacystin and MG132 in normal epithelial cells and in cancer cells. Although loss of cell surface E-cadherin following TGF-beta treatment induced translocation of beta-catenin, an E-cadherin-anchoring molecule, to the nucleus, proteasome inhibition prevented this process and resulted in co-localization of beta-catenin with E-cadherin at the cell surface, leading to establishment of cell-cell adhesion. However, promotion of cell migration by TGF-beta was not significantly affected by proteasome inhibition. Proteasome-dependent events thus appear to be involved in stabilization of cell surface E-cadherin. |
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Authors:
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Masao Saitoh; Takuya Shirakihara; Kohei Miyazono |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-02-24 |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 381 ISSN: 1090-2104 ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2009 Apr |
Date Detail:
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Created Date: 2009-03-30 Completed Date: 2009-04-28 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 560-5 Citation Subset: IM |
Affiliation:
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Department of Molecular Pathology, University of Tokyo, Bunkyo-ku, Japan. msaitoh-ind@umin.ac.jp |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cadherins / genetics, metabolism* Cell Line Cell Membrane / metabolism* Endocytosis Epithelial Cells / cytology, drug effects, metabolism Hepatocyte Growth Factor Mesoderm / cytology, metabolism Mice Proteasome Endopeptidase Complex / antagonists & inhibitors, metabolism* Protein Stability Transforming Growth Factor beta / pharmacology beta Catenin / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Cadherins; 0/Transforming Growth Factor beta; 0/beta Catenin; 67256-21-7/Hepatocyte Growth Factor; EC 3.4.25.1/Proteasome Endopeptidase Complex |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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