Document Detail


Regulation of spatiotemporal expression of cell-cell adhesion molecules during development of Dictyostelium discoideum.
MedLine Citation:
PMID:  21585356     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The social amoeba Dictyostelium discoideum is a simple but powerful model organism for the study of cell-cell adhesion molecules and their role in morphogenesis during development. Three adhesive systems have been characterized and studied in detail. The spatiotemporal expression of these adhesion proteins is stringently regulated, often coinciding with major shifts in the morphological complexity of development. At the onset of development, amoeboid cells express the Ca(2+) -dependent cell-cell adhesion molecule DdCAD-1, which initiates weak homophilic interactions between cells and assists in the recruitment of individuals into cell streams. DdCAD-1 is unique because it is synthesized as a soluble protein in the cytoplasm. It is targeted for presentation on the cell surface by an unconventional protein transport mechanism via the contractile vacuole. Concomitant with the aggregation stage is the expression of the contact sites A glycoprotein csA/gp80 and TgrC1, both of which mediate Ca(2+) /Mg(2+) -independent cell-cell adhesion. Whereas csA/gp80 is a homophilic binding protein, TgrC1 binds to a heterophilic receptor on the cell. During cell aggregation, csA/gp80 associates preferentially with lipid rafts, which facilitate the rapid assembly of adhesion complexes. TgrC1 is synthesized at low levels during aggregation and rapid accumulation occurs initially in the peripheral cells of loose mounds. The extracellular portion of TgrC1 is shed and becomes part of the extracellular matrix. Additionally, analyses of knockout mutants have revealed important biological roles played by these adhesion proteins, including size regulation, cell sorting and cell-type proportioning.
Authors:
Chi-Hung Siu; Shrivani Sriskanthadevan; Jun Wang; Liansheng Hou; Gong Chen; Xiaoqun Xu; Alexander Thomson; Chunxia Yang
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Development, growth & differentiation     Volume:  53     ISSN:  1440-169X     ISO Abbreviation:  Dev. Growth Differ.     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-05-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0356504     Medline TA:  Dev Growth Differ     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  518-27     Citation Subset:  IM    
Copyright Information:
© 2011 The Authors. Development, Growth & Differentiation © 2011 Japanese Society of Developmental Biologists.
Affiliation:
Banting and Best Department of Medical Research and Department of Biochemistry, University of Toronto, Toronto, Ontario M5S 1A8, Canada.
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