| Regulation of sodium-glutamine cotransport in villus and crypt cells by glucocorticoids during chronic enteritis. | |
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MedLine Citation:
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PMID: 22508450 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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BACKGROUND: Assimilation of the preferred nutrient of enterocytes is mediated primarily by sodium (Na)-dependent cotransport (NGct) in the intestine. The predominant NGcT in villus cells, B0AT1, is inhibited secondary to a decrease in cotransporter numbers during chronic intestinal inflammation. In contrast, NGcT mediated by SN2 in crypt cells is stimulated secondary to increased affinity of the cotransporter for glutamine during chronic ileitis. Glucocorticoid is a mainstay of treatment for inflammatory bowel disease. However, its effect on NGcT is not known. METHODS: The inhibition of B0AT1 in villus cells during chronic intestinal inflammation was reversed back to normal by methylprednisolone (MP). This was secondary to the restoration of the cotransporter numbers in the brush border membrane rather than an alteration in the affinity. The stimulation of NGcT in crypt cells during chronic ileitis was also restored back to its normal levels by MP treatment. This reversal was secondary to the restoration of the altered affinity of the cotransporter SN2 for glutamine. RESULTS: Kinetic studies and western blot analysis were consistent with functional studies for both cotransporters. Thus, glucocorticoids restore two uniquely altered Na-glutamine cotransporters, B0AT1 in villus and SN2 in crypt cells during chronic enteritis. CONCLUSIONS: These data indicate that glucocorticoids function as an upstream broad spectrum immune modulator in the chronically inflamed intestine. (Inflamm Bowel Dis 2012;). |
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Authors:
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Subha Arthur; Prosenjit Saha; Shanmuga Sundaram; Ramesh Kekuda; Uma Sundaram |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-4-16 |
Journal Detail:
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Title: Inflammatory bowel diseases Volume: - ISSN: 1536-4844 ISO Abbreviation: - Publication Date: 2012 Apr |
Date Detail:
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Created Date: 2012-4-17 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9508162 Medline TA: Inflamm Bowel Dis Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2012 Crohn's & Colitis Foundation of America, Inc. |
Affiliation:
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Section of Digestive Diseases and Clinical and Translational Science Institute West Virginia University Health Sciences Center, Morgantown, West Virginia. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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