Document Detail

Regulation of ribosomal RNA gene transcription during retinoic acid-induced differentiation of mouse teratocarcinoma cells.
MedLine Citation:
PMID:  9056427     Owner:  NLM     Status:  MEDLINE    
We have examined the mechanism of regulation of rRNA synthesis in mouse F9 teratocarcinoma cells that were induced to differentiate by retinoic acid and dibutyryl cAMP. Ribosomal RNA (rRNA) synthesis was significantly reduced during differentiation of F9 cells into parietal endoderm cells. Nuclear run-on assay revealed that the rRNA gene transcription rates were reduced in differentiated cells, and this phenomenon could be mimicked by in vitro transcription assay using nuclear extracts prepared from F9 stem and F9 parietal endoderm cells. Analysis of the DNA-binding activities of two RNA polymerase I (pol I) transcription factors E1BF/Ku and UBF revealed decreased affinity for their cognate recognition sequences. Immunoblot analysis showed a marked reduction in the amounts of E1BF/Ku and UBF in the differentiated cells. Analysis of the steady-state RNA levels for the smaller subunit of E1BF/Ku and for UBF in differentiating F9 cells revealed decreased mRNA synthesis and increase in message level for the differentiation-specific marker laminin B1 with progression of the differentiated status of the cells. This study has demonstrated that differentiation of mouse F9 teratocarcinoma cells into parietal endoderm cells leads to diminished rRNA synthesis, which may be mediated by reduced DNA-binding activities and amounts of at least two pol I transcription factors.
P K Datta; S Budhiraja; R R Reichel; S T Jacob
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Experimental cell research     Volume:  231     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  1997 Feb 
Date Detail:
Created Date:  1997-03-20     Completed Date:  1997-03-20     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  198-205     Citation Subset:  IM    
Department of Pharmacology and Molecular Biology, The Chicago Medical School, North Chicago, Illinois, 60064, USA.
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MeSH Terms
Antigens, Nuclear*
Bucladesine / pharmacology
Cell Differentiation*
DNA Helicases*
DNA-Binding Proteins / metabolism
Embryonal Carcinoma Stem Cells
Endoderm / cytology
Gene Expression Regulation*
Neoplastic Stem Cells / cytology,  metabolism
Nuclear Proteins / metabolism
Pol1 Transcription Initiation Complex Proteins*
RNA, Messenger / metabolism
RNA, Ribosomal / biosynthesis*
Transcription Factors / metabolism
Transcription, Genetic*
Tretinoin / pharmacology*
Tumor Cells, Cultured
rRNA Operon / genetics*
Grant Support
Reg. No./Substance:
0/Antigens, Nuclear; 0/DNA-Binding Proteins; 0/Ku autoantigen; 0/Nuclear Proteins; 0/Pol1 Transcription Initiation Complex Proteins; 0/RNA, Messenger; 0/RNA, Ribosomal; 0/Transcription Factors; 0/XRCC5 protein, human; 0/transcription factor UBF; 302-79-4/Tretinoin; 362-74-3/Bucladesine; EC 3.6.1.-/DNA Helicases

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