Document Detail


Regulation of replication termination by Reb1 protein-mediated action at a distance.
MedLine Citation:
PMID:  20850009     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
DNA transactions driven by long-range protein-mediated inter- and intrachromosomal interactions have been reported to influence gene expression. Here, we report that site-specific replication termination in Schizosaccharomyces pombe is modulated by protein-mediated interactions between pairs of Ter sites located either on the same or on different chromosomes. The dimeric Reb1 protein catalyzes termination and mediates interaction between Ter sites. The Reb1-dependent interactions between two antiparallel Ter sites in cis caused looping out of the intervening DNA in vitro and enhancement of fork arrest in vivo. A Ter site on chromosome 2 interacted pairwise with two Ter sites located on chromosome 1 by chromosome kissing. Mutational inactivation of the major interacting Ter site on chromosome 1 significantly reduced fork arrest at the Ter site on chromosome 2, thereby revealing a cooperative mechanism of control of replication termination.
Authors:
Samarendra K Singh; Sarah Sabatinos; Susan Forsburg; Deepak Bastia
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Cell     Volume:  142     ISSN:  1097-4172     ISO Abbreviation:  Cell     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-09-20     Completed Date:  2010-10-18     Revised Date:  2014-09-16    
Medline Journal Info:
Nlm Unique ID:  0413066     Medline TA:  Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  868-78     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Inc. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Chromosomes, Fungal
DNA Replication*
DNA-Binding Proteins / metabolism*
Exodeoxyribonucleases / metabolism
Regulatory Sequences, Nucleic Acid
Schizosaccharomyces / metabolism*
Schizosaccharomyces pombe Proteins / metabolism*
Transcription Factors / metabolism*
Grant Support
ID/Acronym/Agency:
GM 049264/GM/NIGMS NIH HHS; GM 049264-17S1/GM/NIGMS NIH HHS; P41 RR011823/RR/NCRR NIH HHS; P41 RR011823-148054/RR/NCRR NIH HHS; R01 GM049264/GM/NIGMS NIH HHS; R01 GM049264-17/GM/NIGMS NIH HHS; R01 GM049264-17S1/GM/NIGMS NIH HHS; R01 GM059321/GM/NIGMS NIH HHS; R01 GM059321-10/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/DNA-Binding Proteins; 0/Schizosaccharomyces pombe Proteins; 0/Transcription Factors; 0/reb1 protein, S pombe; EC 3.1.-/Exodeoxyribonucleases; EC 3.1.11.2/exodeoxyribonuclease III
Comments/Corrections

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